Abbara Chadi, Galy Romain, Benyamina Amine, Reynaud Michel, Bonhomme-Faivre Laurence
Laboratoire de Pharmacologie, Service de Pharmacie, Hôpital Paul BROUSSE (AP-HP), 12, Avenue Paul Vaillant Couturier, 94800 Villejuif, France.
J Pharm Biomed Anal. 2006 Jun 7;41(3):1011-6. doi: 10.1016/j.jpba.2006.01.017. Epub 2006 Feb 24.
A high performance liquid chromatography (HPLC) procedure for the determination of Delta9 tetrahydrocannabinol (THC) in human plasma is described. A two-step solid-phase extraction on CN cartridges was coupled with a reversed phase HPLC system. THC was eluted using a mobile phase composed of methanol, acetonitrile and tetrabutylammonium perchlorate solution (0.005 M, pH 3.2), through a C18 Nucleosil column and detected at a wavelength of 215 nm. Calibration curve was linear over the range 5-100 ng/ml with a lower limit of quantification validated at 5 ng/ml. Extraction recovery using the developed extraction procedure was higher than 85%. This method is presently used for the quantification of THC in plasma samples from regular cannabis smokers.
描述了一种用于测定人血浆中Δ⁹-四氢大麻酚（THC）的高效液相色谱（HPLC）方法。在CN柱上进行两步固相萃取，并与反相HPLC系统联用。通过C18 Nucleosil柱，使用由甲醇、乙腈和高氯酸四丁铵溶液（0.005 M，pH 3.2）组成的流动相洗脱THC，并在215 nm波长处进行检测。校准曲线在5 - 100 ng/ml范围内呈线性，定量下限验证为5 ng/ml。使用所开发的萃取方法，萃取回收率高于85%。该方法目前用于定量常规大麻吸烟者血浆样本中的THC。
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