Mercolini Laura, Musenga Alessandro, Comin Irene, Baccini Cesare, Conti Matteo, Raggi Maria Augusta
Laboratory of Pharmaco-Toxicological Analysis, Department of Pharmaceutical Sciences, Faculty of Pharmacy, Alma Mater Studiorum-University of Bologna, Via Belmeloro 6, I-40126 Bologna, Italy.
J Pharm Biomed Anal. 2008 May 12;47(1):156-63. doi: 10.1016/j.jpba.2007.12.023. Epub 2007 Dec 23.
Delta9-Tetrahydrocannabinol is the most widespread drug of abuse in the world and it is also currently available as the active principle of formulations for the treatment of chronic pain. Its main metabolite, 11-nor-Delta9-tetrahydrocannabinol-9-carboxylic acid, is the most important marker of Delta9-tetrahydrocannabinol consumption. An original liquid chromatographic method has been developed for the determination of these two analytes in human plasma and urine. Separation was obtained on a C8 column using a mobile phase with 35% phosphate buffer at pH 2.7 and 65% acetonitrile. The UV detector was set at 220 nm and indomethacin was used as the internal standard. Sample pre-treatment was carried out by solid-phase extraction with C8 cartridges; urine samples were subjected to basic hydrolysis before extraction. Both extraction yields (>91%) and precision values were highly satisfactory. The method was successfully applied to biological samples collected from Cannabis users. Accuracy and selectivity results were satisfactory. This is the first HPLC-UV method developed for the simultaneous quantification of Delta9-tetrahydrocannabinol and 11-nor-Delta9-tetrahydrocannabinol-9-carboxylic acid in both plasma and urine for the monitoring of either therapeutic or recreational use.
Δ9-四氢大麻酚是世界上滥用最为广泛的毒品，目前它也是治疗慢性疼痛制剂的活性成分。其主要代谢物11-去甲-Δ9-四氢大麻酚-9-羧酸是Δ9-四氢大麻酚消费的最重要标志物。已开发出一种原创的液相色谱法，用于测定人血浆和尿液中的这两种分析物。在C8柱上进行分离，使用pH值为2.7的35%磷酸盐缓冲液和65%乙腈的流动相。紫外检测器设置在220 nm，吲哚美辛用作内标。通过使用C8小柱进行固相萃取进行样品预处理；尿液样品在萃取前进行碱性水解。萃取产率（>91%）和精密度值都非常令人满意。该方法成功应用于从大麻使用者收集的生物样品。准确度和选择性结果令人满意。这是首个开发的用于同时定量血浆和尿液中Δ9-四氢大麻酚和11-去甲-Δ9-四氢大麻酚-9-羧酸以监测治疗用途或娱乐用途的高效液相色谱-紫外方法。
