IS50内侧末端的一个序列下调转座作用。

A sequence at the inside end of IS50 down regulates transposition.

作者信息

Dodson K W, Berg D E

机构信息

Washington University Medical School, Department of Molecular Microbiology, St. Louis, Missouri 63110-1093.

出版信息

Plasmid. 1991 Mar;25(2):145-8. doi: 10.1016/0147-619x(91)90028-u.

DOI:10.1016/0147-619x(91)90028-u

PMID:1650007

Abstract

Deletion analysis has shown that the segment at the IS50 inside (I) end that is needed for efficient transposition is approximately 19 bp long. Dam methylation at two 5' GATC sequences within this segment decreases I-end transposition activity. A third 5' GATC sequence is present at bp 21-24 of the I end. The comparisons presented here show that extension of the I end from 19 to 24 bp decreases its transposition activity in dam cells 5- to 50-fold, depending on the overall transposon structure.

摘要

缺失分析表明，高效转座所需的位于IS50内侧（I）末端的片段长度约为19bp。该片段内两个5' GATC序列处的Dam甲基化会降低I末端的转座活性。I末端的第21 - 24bp处存在第三个5' GATC序列。此处进行的比较表明，将I末端从19bp延长至24bp会使dam细胞中的转座活性降低5至50倍，具体倍数取决于整体转座子结构。