IS50转座酶基因的定位与IS50转座
Orientation of IS50 transposase gene and IS50 transposition.
作者信息
Makris J C, Reznikoff W S
机构信息
Department of Biochemistry, College of Agricultural and Life Science, University of Wisconsin-Madison 53706.
出版信息
J Bacteriol. 1989 Sep;171(9):5212-4. doi: 10.1128/jb.171.9.5212-5214.1989.
Abstract
Reversal of transposase gene orientation with respect to the nonidentical ends of IS50 strongly decreased IS50 transposition in both Dam- and Dam+ hosts. In either orientation, IS50 transposase expression was unaffected. These effects were independent of the surrounding DNA context. This shows that the efficiency of IS50 transposition is dependent on transposase gene orientation. The transposition frequencies of transposons utilizing inverted IS50 inside ends (IE), IE-IE transposons, were lower than either outside end (OE)-IE or OE-OE transposons.
摘要
相对于IS50的不相同末端，转座酶基因方向的反转在Dam-和Dam+宿主中均强烈降低了IS50转座。在任何一种方向上，IS50转座酶的表达均不受影响。这些效应独立于周围的DNA环境。这表明IS50转座的效率取决于转座酶基因的方向。利用反向IS50内侧末端（IE）的转座子（即IE-IE转座子）的转座频率低于外侧末端（OE）-IE或OE-OE转座子。
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引用本文的文献
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2
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本文引用的文献
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