Suppr超能文献

膜联蛋白2:一种新型的1型人类免疫缺陷病毒Gag结合蛋白,参与单核细胞衍生巨噬细胞中的复制。

Annexin 2: a novel human immunodeficiency virus type 1 Gag binding protein involved in replication in monocyte-derived macrophages.

作者信息

Ryzhova Elena V, Vos Robin M, Albright Andrew V, Harrist Alexia V, Harvey Thomas, González-Scarano Francisco

机构信息

Department of Neurology and Microbiology, University of Pennsylvania, 3 W. Gates, 3400 Spruce Street, Philadelphia, Pennsylvania 19104-4283, USA.

出版信息

J Virol. 2006 Mar;80(6):2694-704. doi: 10.1128/JVI.80.6.2694-2704.2006.

Abstract

Human immunodeficiency virus (HIV) replication in the major natural target cells, CD4+ T lymphocytes and macrophages, is parallel in many aspects of the virus life cycle. However, it differs as to viral assembly and budding, which take place on plasma membranes in T cells and on endosomal membranes in macrophages. It has been postulated that cell type-specific host factors may aid in directing viral assembly to distinct destinations. In this study we defined annexin 2 (Anx2) as a novel HIV Gag binding partner in macrophages. Anx2-Gag binding was confined to productively infected macrophages and was not detected in quiescently infected monocyte-derived macrophages (MDM) in which an HIV replication block was mapped to the late stages of the viral life cycle (A. V. Albright, R. M. Vos, and F. Gonzalez-Scarano, Virology 325:328-339, 2004). We demonstrate that the Anx2-Gag interaction likely occurs at the limiting membranes of late endosomes/multivesicular bodies and that Anx2 depletion is associated with a significant decline in the infectivity of released virions; this coincided with incomplete Gag processing and inefficient incorporation of CD63. Cumulatively, our data suggest that Anx2 is essential for the proper assembly of HIV in MDM.

摘要

人类免疫缺陷病毒(HIV)在主要天然靶细胞,即CD4 + T淋巴细胞和巨噬细胞中的复制,在病毒生命周期的许多方面是并行的。然而,在病毒组装和出芽方面存在差异,这分别发生在T细胞的质膜上和巨噬细胞的内体膜上。据推测,细胞类型特异性宿主因子可能有助于将病毒组装引导至不同的目的地。在本研究中,我们将膜联蛋白2(Anx2)定义为巨噬细胞中一种新的HIV Gag结合伴侣。Anx2与Gag的结合仅限于高效感染的巨噬细胞,而在静止感染的单核细胞衍生巨噬细胞(MDM)中未检测到,在这些细胞中,HIV复制阻滞被定位到病毒生命周期的后期(A. V. Albright、R. M. Vos和F. Gonzalez-Scarano,《病毒学》325:328 - 339,2004)。我们证明Anx2与Gag的相互作用可能发生在晚期内体/多囊泡体的限制膜上,并且Anx2的缺失与释放病毒颗粒的感染性显著下降有关;这与Gag加工不完全和CD63掺入效率低下相一致。总的来说,我们的数据表明Anx2对于MDM中HIV的正确组装至关重要。

相似文献

5
Infectious HIV-1 assembles in late endosomes in primary macrophages.
J Cell Biol. 2003 Aug 4;162(3):443-55. doi: 10.1083/jcb.200304008. Epub 2003 Jul 28.
7
Human Discs Large is a new negative regulator of human immunodeficiency virus-1 infectivity.
Mol Biol Cell. 2009 Jan;20(1):498-508. doi: 10.1091/mbc.e08-02-0189. Epub 2008 Oct 22.
8
Inhibition or deficiency of cathepsin B leads defects in HIV-1 Gag pseudoparticle release in macrophages and HEK293T cells.
Antiviral Res. 2012 Jan;93(1):175-84. doi: 10.1016/j.antiviral.2011.11.009. Epub 2011 Nov 26.
9
Exosomes and HIV Gag bud from endosome-like domains of the T cell plasma membrane.
J Cell Biol. 2006 Mar 13;172(6):923-35. doi: 10.1083/jcb.200508014.
10
Assembly of infectious HIV-1 in human epithelial and T-lymphoblastic cell lines.
J Mol Biol. 2006 Jun 16;359(4):848-62. doi: 10.1016/j.jmb.2006.04.017. Epub 2006 Apr 25.

引用本文的文献

1
Negative interplay between HIV-1 Gag and amyloid precursor protein centers around competition for VPS4A and TSG101.
Proc Natl Acad Sci U S A. 2025 Aug 26;122(34):e2503988122. doi: 10.1073/pnas.2503988122. Epub 2025 Aug 21.
2
Annexin, a Protein for All Seasons: From Calcium Dependent Membrane Metabolism to RNA Recognition.
Bioessays. 2025 Jul;47(7):e70019. doi: 10.1002/bies.70019. Epub 2025 May 12.
3
Annexin A2: the missing piece in the puzzle of pathogen-induced damage.
Virulence. 2023 Dec;14(1):2237222. doi: 10.1080/21505594.2023.2237222.
8
Annexin A2 in Virus Infection.
Front Microbiol. 2018 Dec 5;9:2954. doi: 10.3389/fmicb.2018.02954. eCollection 2018.
9
Mass spectrometry-based investigation of measles and mumps virus proteome.
Virol J. 2018 Oct 16;15(1):160. doi: 10.1186/s12985-018-1073-9.
10
Annexin A2 Mediates the Localization of Measles Virus Matrix Protein at the Plasma Membrane.
J Virol. 2018 Apr 27;92(10). doi: 10.1128/JVI.00181-18. Print 2018 May 15.

本文引用的文献

1
3
Retrovirus budding.
Annu Rev Cell Dev Biol. 2004;20:395-425. doi: 10.1146/annurev.cellbio.20.010403.102350.
4
Phosphatidylinositol (4,5) bisphosphate regulates HIV-1 Gag targeting to the plasma membrane.
Proc Natl Acad Sci U S A. 2004 Oct 12;101(41):14889-94. doi: 10.1073/pnas.0405596101. Epub 2004 Oct 1.
7
Human immunodeficiency virus type 1 infection induces cyclin T1 expression in macrophages.
J Virol. 2004 Aug;78(15):8114-9. doi: 10.1128/JVI.78.15.8114-8119.2004.
9
Annexin 2 is a phosphatidylinositol (4,5)-bisphosphate binding protein recruited to actin assembly sites at cellular membranes.
J Cell Sci. 2004 Jul 15;117(Pt 16):3473-80. doi: 10.1242/jcs.01208. Epub 2004 Jun 29.
10
Annexins--unique membrane binding proteins with diverse functions.
J Cell Sci. 2004 Jun 1;117(Pt 13):2631-9. doi: 10.1242/jcs.01245.

文献AI研究员

20分钟写一篇综述,助力文献阅读效率提升50倍。

立即体验

用中文搜PubMed

大模型驱动的PubMed中文搜索引擎

马上搜索

文档翻译

学术文献翻译模型,支持多种主流文档格式。

立即体验