Hancer Veysel Sabri, Diz-Kucukkaya Reyhan, Bilge Ahmet Kaya, Ozben Beste, Oncul Aytac, Ergen Gursel, Nalcaci Meliha
Division of Hematology, Department of Internal Medicine, Istanbul Faculty of Medicine, Turkey.
Circ J. 2006 Mar;70(3):239-42. doi: 10.1253/circj.70.239.
Activated factor XIII (FXIII) cross-links between fibrin monomers, thus increasing the clot stability and resistance to fibrinolysis. Congenital FXIII deficiency causes severe bleeding diathesis. Recently, a common polymorphism of the FXIII A subunit (FXIII Val34Leu) has been identified as a protective factor against both arterial and venous thrombosis. The aim of this study was to investigate the role of FXIII Val34Leu polymorphism in coronary artery thrombosis, especially in young patients.
One hundred and thirty patients under than 60 years of age with a history of myocardial infarction (%) and 130 healthy control subjects in the same age group were included to our study. Genomic DNA was extracted from venous blood samples and the polymerase chain reaction method was used to genotype FXIII Val34Leu polymorphism. Coronary risk factors such as obesity, diabetes mellitus, hyperlipidemia and smoking were compared between the groups with chi-square test and logistic regression analysis. The Leu allele frequency was significantly lower in patient group compared to control group (7.69% vs 19.23%, p=0.0001, chi-square). This difference was extremely significant in patients younger than 50 years-old (5.26% vs 19.64%, p<0.0001, chi-square).
Our findings support the hypothesis that Val34Leu polymorphism in FXIII gene has a protective effect against myocardial infarction.
活化的因子 XIII（FXIII）在纤维蛋白单体之间形成交联，从而增加凝块稳定性和对纤维蛋白溶解的抵抗力。先天性 FXIII 缺乏会导致严重的出血素质。最近，FXIII A 亚基的一种常见多态性（FXIII Val34Leu）已被确定为预防动脉和静脉血栓形成的保护因素。本研究的目的是探讨 FXIII Val34Leu 多态性在冠状动脉血栓形成中的作用，特别是在年轻患者中。
本研究纳入了 130 例年龄小于 60 岁且有心肌梗死病史的患者以及 130 例同年龄组的健康对照者。从静脉血样本中提取基因组 DNA，并采用聚合酶链反应方法对 FXIII Val34Leu 多态性进行基因分型。采用卡方检验和逻辑回归分析比较两组之间的冠状动脉危险因素，如肥胖、糖尿病、高脂血症和吸烟情况。患者组的 Leu 等位基因频率显著低于对照组（7.69% 对 19.23%，p = 0.0001，卡方检验）。这种差异在年龄小于 50 岁的患者中极为显著（5.26% 对 19.64%，p < 0.0001，卡方检验）。
我们的研究结果支持以下假设，即 FXIII 基因中的 Val34Leu 多态性对心肌梗死具有保护作用。
