El-Akawi Zeyad J, Nusier Mohamad K, Zoughool Foad E
Department of Biochemistry and Molecular Biology, Faculty of Medicine, Jordan University of Science and Technology, Irbid.
Saudi Med J. 2006 Feb;27(2):181-4.
Alpha-1 antitrypsin (alpha1-AT) is a secretory glycoprotein produced mainly in the liver and monocytes. It is the most abundant serine protease inhibitor in human plasma. It predominantly inhibits neutrophil elastase thus, it prevents the breakdown of lung tissue. The deficiency of alpha1-AT is an inherited disorder characterized by reduced serum level of alpha1-AT. Protease inhibitors Z (PiZ) and protease inhibitors S (PiS) are the most common deficient genotypes of alpha1-AT. The aim of this study is to test the relationship between alpha1-AT deficient genotypes S and Z and lung cancer in Jordanian lung cancer patients.
We obtained the samples used in this study from 100 paraffin embedded tissue blocks of the lung cancer patients from Prince Iman Research Center and Laboratory Sciences at King Hussein Medical Center, Amman, Jordan. Analyses of the Z and S genotypes of alpha1-AT were performed by polymerase chain reaction and restriction fragment length polymorphism techniques at Jordan University of Science and Technology during 2003 and 2004.
We demonstrated that all lung cancer patients were of M genotype, and no Z or S genotypes were detected.
There is no relationship between alpha1-AT deficient genotypes S and Z and lung cancer in patients involved in this study.
α1抗胰蛋白酶（α1-AT）是一种主要在肝脏和单核细胞中产生的分泌性糖蛋白。它是人类血浆中最丰富的丝氨酸蛋白酶抑制剂。它主要抑制中性粒细胞弹性蛋白酶，从而防止肺组织分解。α1-AT缺乏是一种遗传性疾病，其特征是血清α1-AT水平降低。蛋白酶抑制剂Z（PiZ）和蛋白酶抑制剂S（PiS）是α1-AT最常见的缺陷基因型。本研究的目的是测试约旦肺癌患者中α1-AT缺陷基因型S和Z与肺癌之间的关系。
我们从约旦安曼侯赛因国王医疗中心伊曼王子研究中心和实验室科学部的100例肺癌患者石蜡包埋组织块中获取了本研究使用的样本。2003年至2004年期间，在约旦科技大学通过聚合酶链反应和限制性片段长度多态性技术对α1-AT的Z和S基因型进行了分析。
我们证明所有肺癌患者均为M基因型，未检测到Z或S基因型。
本研究涉及的患者中，α1-AT缺陷基因型S和Z与肺癌之间没有关系。
