Ueda M, Taketa K, Kosaka K
Clin Chim Acta. 1975 Apr 2;60(1):77-84. doi: 10.1016/0009-8981(75)90182-5.
Activities of hexokinase isozymes in carbon tetrachloride (CCl(4))-injured rat liver were determined quantitatively by DEAE-cellulose column chromatography and compared with those of regenerating liver, fetal liver and ascites hepatoma cells (AH 130). The CCl(4)-injured liver revealed an isozyme distribution with predominant Types I, II and III (3.2, 8.8 and 6.8 times higher than the control values, respectively) and with undetectable activity of Type IV hexokinase (glucokinase). Although the isozyme pattern generally resembled that of fetal liver or hepatoma cells, the relatively high activity of hexokinase Type III in CCl(4) treatment characterizes the pattern of hexokinase isozyme in acue liver damage.
通过DEAE-纤维素柱色谱法定量测定了四氯化碳（CCl₄）损伤的大鼠肝脏中己糖激酶同工酶的活性，并与再生肝脏、胎儿肝脏和腹水肝癌细胞（AH 130）的活性进行了比较。CCl₄损伤的肝脏显示出同工酶分布，其中I型、II型和III型占主导（分别比对照值高3.2倍、8.8倍和6.8倍），IV型己糖激酶（葡萄糖激酶）活性未检测到。尽管同工酶模式总体上类似于胎儿肝脏或肝癌细胞的模式，但CCl₄处理中己糖激酶III型的相对高活性是急性肝损伤中己糖激酶同工酶模式的特征。
