Gobeev V N, Khripach L V
Biokhimiia. 1976 Aug;41(8):1504-9.
Electrophoretic study of hexokinase (HK) associated with the soluble fraction of mouse transplantable hepatoma 22a revealed that almost all bands of HK activities overlapped the bands of glucose-6-P dehydrogenase (G6PDH) activities in the gels. Similar results were obtained for liver, muscle and brain soluble fractions, as well as for various extracts from hepatoma 22a mitochondria and commercial preparation of yeast HK. A single type of HK, which does not overlap G6PDH activity, was located between types I and II (according to the Katzen classification) as a diffuse band of 1 hour manifestation. A possibility of structural organization of glycolytic enzymes in the cell essential for the quantitative estimation of the isozyme pattern is discussed.
对与小鼠可移植性肝癌22a可溶性部分相关的己糖激酶（HK）进行的电泳研究表明，在凝胶中，HK活性的几乎所有条带都与葡萄糖-6-磷酸脱氢酶（G6PDH）活性的条带重叠。肝脏、肌肉和脑可溶性部分，以及肝癌22a线粒体的各种提取物和酵母HK的商业制剂也得到了类似结果。一种不与G6PDH活性重叠的单一类型的HK，位于I型和II型之间（根据卡曾分类法），表现为1小时的弥散条带。讨论了细胞中糖酵解酶结构组织对于同工酶模式定量估计的必要性。
