表皮生长因子和卡巴胆碱对胰腺腺泡细胞中磷酸肌醇合成与分解的比较作用

Comparative effects of epidermal growth factor and carbachol on phosphoinositide synthesis and breakdown in pancreatic acinar cells.

作者信息

Conway B R, Laychock S G, Rubin R P

机构信息

Department of Pharmacology and Toxicology, Medical College of Virginia, Richmond 23298.

出版信息

Biochem Biophys Res Commun. 1991 Jul 31;178(2):780-5. doi: 10.1016/0006-291x(91)90176-8.

DOI:10.1016/0006-291x(91)90176-8

PMID:1650199

Abstract

Carbachol (CCh) and epidermal growth factor (EGF) elicited a concentration-dependent increase in [32P]phosphatidyl-inositol-4-phosphate (PtdIns-4P) formation in homogenates derived from agonist-stimulated rat pancreatic acini. The combination of CCh and EGF produced a response which was not synergistic or additive. EGF, unlike CCh, failed to cause [32P]PtdIns-4,5P2 breakdown, suggesting different mechanisms involved in the stimulation of [32P]PtdIns-4P formation induced by EGF and CCh. We conclude that PtdIns kinase represents a key component of the signaling pathways utilized by EGF and CCh in exocrine pancreas.

摘要

卡巴胆碱（CCh）和表皮生长因子（EGF）可使来自激动剂刺激的大鼠胰腺腺泡的匀浆中[32P]磷脂酰肌醇-4-磷酸（PtdIns-4P）的生成呈浓度依赖性增加。CCh和EGF的联合作用产生的反应既无协同性也无加和性。与CCh不同，EGF未能引起[32P]PtdIns-4,5P2的分解，这表明EGF和CCh诱导[32P]PtdIns-4P生成的刺激机制不同。我们得出结论，磷脂酰肌醇激酶是EGF和CCh在外分泌胰腺中利用的信号通路的关键组成部分。