Raugei G, Marzocchini R, Modesti A, Ratti G, Cappugi G, Camici G, Manao G, Ramponi G
Department of Biochemical Sciences, University of Florence, Italy.
Biochem Int. 1991 Jan;23(2):317-26.
A chemically synthesized polynucleotide sequence coding for bovine liver low molecular weight acid phosphatase (which possesses phosphotyrosine-protein phosphatase activity) has been cloned in a E. coli expression vector. The recombinant protein retains correct affinity for substrate and inhibitors but shows a reduced specific activity.
