用于在线、时间分辨化学监测的快速哈达玛变换毛细管电泳

Fast Hadamard transform capillary electrophoresis for on-line, time-resolved chemical monitoring.

作者信息

Braun Kevin L, Hapuarachchi Suminda, Fernandez Facundo M, Aspinwall Craig A

机构信息

Department of Chemistry, University of Arizona, Tucson, Arizona 85721, USA.

出版信息

Anal Chem. 2006 Mar 1;78(5):1628-35. doi: 10.1021/ac051710w.

DOI:10.1021/ac051710w

PMID:16503616

Abstract

We report a new approach for collecting and deconvoluting the data in Hadamard transform capillary electrophoresis, referred to as fast Hadamard transform capillary electrophoresis (fHTCE). Using fHTCE, total analysis times can be reduced by up to 48% per multiplexed separation compared to conventional Hadamard transform capillary electrophoresis (cHTCE) while providing comparable signal-to-noise ratio enhancements. In fHTCE, the sample is injected following a pseudorandom pulsing sequence derived from the first row of a simplex matrix (S-matrix) in contrast to cHTCE, which utilizes a sequence of twice the length. In addition to the temporal savings provided by fHTCE, a 50% reduction in sample consumption is also realized due to the decreased number of sample injections. We have applied fHTCE to the analysis of mixtures of neurotransmitters and related compounds to yield improved signal-to-noise ratios with a total analysis time under 10 s. In addition, we demonstrate the capability of fHTCE to perform time-resolved monitoring of changes in the concentration of model neurochemical compounds.

摘要

我们报道了一种在哈达玛变换毛细管电泳中收集和解卷积数据的新方法，称为快速哈达玛变换毛细管电泳（fHTCE）。与传统哈达玛变换毛细管电泳（cHTCE）相比，使用fHTCE时，每次多重分离的总分析时间最多可减少48%，同时提供相当的信噪比增强效果。在fHTCE中，样品按照从单纯形矩阵（S矩阵）第一行导出的伪随机脉冲序列进样，而cHTCE则使用长度为其两倍的序列。除了fHTCE节省的时间外，由于进样次数减少，样品消耗也减少了50%。我们已将fHTCE应用于神经递质及相关化合物混合物的分析，以在总分析时间不到10秒的情况下获得更高的信噪比。此外，我们展示了fHTCE对模型神经化学化合物浓度变化进行时间分辨监测的能力。