Wu Ted H, Cañas Jaclyn E, Rainwater Thomas R, Platt Steven G, McMurry Scott T, Anderson Todd A
Department of Chemistry and Biochemistry, University of Alaska Fairbanks, Fairbanks, AK 99775-6160, USA.
Environ Pollut. 2006 Nov;144(1):151-7. doi: 10.1016/j.envpol.2005.12.021. Epub 2006 Feb 28.
Seven complete clutches of Morelet's crocodile (Crocodylus moreletii) eggs were collected in northern Belize and examined for organochlorine (OC) pesticide residues. The primary OC detected, p,p-DDE, was found in every egg analyzed (n=175). Other OCs detected included p,p-DDT, p,p-DDD, methoxychlor, aldrin, and endosulfan I. Concentrations of individual OCs ranged from 4 ppb (ng chemical/g egg wet weight) to greater than 500 ppb. A statistical evaluation of p,p-DDE levels in three complete clutches was used to derive the minimum number of eggs needed from a clutch to precisely determine the mean p,p-DDE concentration representative of that clutch. Sample sizes of 8 (80% confidence level) and 11 (90% confidence level) were determined to yield an accurate estimate of contaminant levels in a full clutch of eggs. The statistically recommended sample size of 11 eggs (at 90% confidence level) was successfully tested on the four additional clutches.
在伯利兹北部收集了七窝完整的莫雷莱鳄（Crocodylus moreletii）蛋，并检测了其中有机氯（OC）农药残留情况。所检测到的主要有机氯成分p,p-DDE，在每一枚被分析的蛋中（n = 175）都有发现。检测到的其他有机氯包括p,p-DDT、p,p-DDD、甲氧滴滴涕、艾氏剂和硫丹I。各有机氯的浓度范围为4 ppb（纳克化学物质/克蛋湿重）至超过500 ppb。通过对三窝完整蛋中p,p-DDE水平进行统计评估，得出为精确确定代表该窝蛋的p,p-DDE平均浓度所需的最少蛋数。确定样本量为8个（80%置信水平）和11个（90%置信水平）时，能够准确估计一窝完整蛋中的污染物水平。针对另外四窝蛋成功测试了统计学上建议的11枚蛋的样本量（90%置信水平）。
