从富含锌的土壤中分离出的卷缘桩菇菌株中，液泡和线粒体的运动性及管状结构响应锌的变化。
Changes in vacuolar and mitochondrial motility and tubularity in response to zinc in a Paxillus involutus isolate from a zinc-rich soil.
作者信息
Tuszynska Sandra, Davies Danielle, Turnau Katarzyna, Ashford Anne E
机构信息
School of Biological, Earth and Environmental Sciences, The University of New South Wales, Sydney, Australia.
出版信息
Fungal Genet Biol. 2006 Mar;43(3):155-63. doi: 10.1016/j.fgb.2005.11.004. Epub 2006 Feb 28.
Short-term effects of zinc on organelles were investigated in Paxillus involutus from a zinc-rich soil. Vacuoles were labelled with Oregon Green 488 carboxylic acid and mitochondria with DiOC(6)(3). Hyphae were treated with ZnSO(4) in the range 1-100 mM and examined by fluorescence microscopy. ZnSO(4) caused loss of tubularity and motility in both organelles depending on concentration and exposure time. Tubular vacuoles thickened after 15 min in 5 mM ZnSO(4) and became spherical at higher concentrations. Mitochondria fragmented after 30 min in 25 mM ZnSO(4). Vacuoles recovered their tubularity after transfer to reverse osmosis water depending on ZnSO(4) concentration and exposure time during treatment. Mitochondria recovered their tubularity with time, both with and without removal of the ZnSO(4) solution. K(2)SO(4) (as control) had no effect on vacuoles but disrupted mitochondria, the effect also depending on concentration and duration of exposure.
研究了锌对来自富锌土壤的卷缘桩菇细胞器的短期影响。用 Oregon Green 488 羧酸标记液泡，用 DiOC(6)(3) 标记线粒体。用 1 - 100 mM 范围内的 ZnSO₄ 处理菌丝，并通过荧光显微镜检查。ZnSO₄ 根据浓度和暴露时间导致两种细胞器的管状结构丧失和运动性丧失。在 5 mM ZnSO₄ 中处理 15 分钟后，管状液泡变厚，在更高浓度下变为球形。在 25 mM ZnSO₄ 中处理 30 分钟后线粒体碎片化。根据处理期间的 ZnSO₄ 浓度和暴露时间，转移到反渗透水中后，液泡恢复了它们的管状结构。无论是否去除 ZnSO₄ 溶液，线粒体都会随着时间恢复其管状结构。K₂SO₄（作为对照）对液泡没有影响，但会破坏线粒体，其影响也取决于浓度和暴露持续时间。
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