Hori Toshihiko, Hashimoto Hiroshi, Konishi Masaharu
Discovery Research Laboratories, Shionogi & Co. Ltd, 3-1-1 Futaba-cho, Toyonaka, Osaka 561-0825, Japan.
Biomed Chromatogr. 2006 Sep;20(9):917-23. doi: 10.1002/bmc.619.
A simple and sensitive method was developed for the determination of erythromycin A (EA), decladinosyl erythromycin A (dClEA) and erythromycin B (EB) in rat plasma and urine by high-performance liquid chromatography with electrogenerated chemiluminescence detection using Tris(2,2'-bipyridine)ruthenium(II). The recovery rates of EA, dClEA and EB were 97, 94 and 85% from rat plasma and 89, 83 and 93% from rat urine, respectively. The calibration curves were linear over the concentration ranges 0.05-5 microg/mL for plasma and 0.5-50 microg/mL for urine. The precision and accuracy for all analytes in rat plasma were < or =9.0 and -6.3-7.2%, and those in urine were < or =9.4% and -6.1-7.6%, respectively. This method proved to be a powerful tool for determination of EA, dClEA and EB concentrations in samples from rats.
建立了一种简单灵敏的方法，采用基于三（2,2'-联吡啶）钌（II）的电致化学发光检测的高效液相色谱法测定大鼠血浆和尿液中的红霉素A（EA）、去克拉定糖红霉素A（dClEA）和红霉素B（EB）。EA、dClEA和EB从大鼠血浆中的回收率分别为97%、94%和85%，从大鼠尿液中的回收率分别为89%、83%和93%。血浆浓度范围在0.05 - 5 μg/mL、尿液浓度范围在0.5 - 50 μg/mL时，校准曲线呈线性。大鼠血浆中所有分析物的精密度和准确度分别≤9.0%和 - 6.3 - 7.2%，尿液中分别≤9.4%和 - 6.1 - 7.6%。该方法被证明是测定大鼠样品中EA、dClEA和EB浓度的有力工具。
