Martínez-Toledo A, Ríos-Leal E, Vázquez-Duhalt R, González-Chávez M del C, Esparza-García J F, Rodríguez-Vázquez R
Centro de Investigación y Estudios Avanzados del IPN, Av IPN No. 2508, Col. San Pedro Zacatenco, CP 07360, México, DF.
Environ Technol. 2006 Feb;27(2):137-42. doi: 10.1080/09593332708618628.
The role of phenanthrene in rhamnolipid production by P. putida in eight media with different culture conditions was investigated. Cultures using Fe2SO4.7H2O, KH2PO4, NH4Cl, yeast extract, glucose, and corn oil, with and without 200 mg l(-1) of phenanthrene, were evaluated under shaking for rhamnolipid production through a 2(7-4) fractional factorial experimental design. The biosurfactant production, decrease in the surface tension of the broth and the total cell growth in media without phenanthrene were affected significantly (p < 0.001) by yeast extract, glucose, corn oil and NH4Cl, and in media with phenanthrene by glucose and yeast extract. The non polar fraction of the biosurfactant in all media was composed of linoleic (C18:2), arachidic (C20:0) and behenic (C22:0) fatty acids. The medium with phenanthrene (200 mg l(-1)), Fe2SO4.7H2O (5 x 10(-4) g l(-1)), KH2PO4 (0.2 g l(-1)), glucose (50 g l(-1)), yeast extract (1 g l(-1)), corn oil (2% vol), and NH4Cl (1 g l(-1)), shaken at 150 rpm at 37 degrees C, and pH 7.0, presented the highest biosurfactant production. For this medium the surface tension decreased by 35.9 mN m(-1) in relation to the initial value, and only this medium showed an emulsion capacity of 20%. The polar fraction (Rhamnose) in media 1, 3, 7 and 8 with phenanthrene was c.a 100%, in contrast to those without phenanthrene where this fraction was undetectable.
研究了菲在不同培养条件下的8种培养基中对恶臭假单胞菌产鼠李糖脂的作用。通过2(7-4)部分因子实验设计，对使用Fe2SO4·7H2O、KH2PO4、NH4Cl、酵母提取物、葡萄糖和玉米油，添加和不添加200 mg l(-1)菲的培养物在振荡条件下产鼠李糖脂的情况进行了评估。在无菲的培养基中，酵母提取物、葡萄糖、玉米油和NH4Cl对生物表面活性剂的产生、肉汤表面张力的降低和总细胞生长有显著影响(p < 0.001)；在含菲的培养基中，葡萄糖和酵母提取物有显著影响。所有培养基中生物表面活性剂的非极性部分由亚油酸(C18:2)、花生酸(C20:0)和山嵛酸(C22:0)脂肪酸组成。含菲(200 mg l(-1))、Fe2SO4·7H2O(5×10(-4) g l(-1))、KH2PO4(0.2 g l(-1))、葡萄糖(50 g l(-1))、酵母提取物(1 g l(-1))、玉米油(2%体积分数)和NH4Cl(1 g l(-1))的培养基，在37℃、150 rpm振荡、pH 7.0条件下，生物表面活性剂产量最高。对于该培养基，表面张力相对于初始值降低了35.9 mN m(-1)，且只有该培养基显示出20%的乳化能力。含菲的培养基1、3、7和8中的极性部分(鼠李糖)约为100%，而无菲的培养基中该部分无法检测到。
