State Key Laboratory of Water Resources and Hydropower Engineering Science, Wuhan University, Wuhan, Hubei 430072, China; Key Laboratory for Algal Biology, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, China.
Key Laboratory for Algal Biology, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, China.
Ecotoxicol Environ Saf. 2021 Jan 1;207:111514. doi: 10.1016/j.ecoenv.2020.111514. Epub 2020 Oct 29.
The present study investigated the stability and efficacy of a biosurfactant produced by Klebsiella sp. KOD36 under extreme conditions and its potential for enhancing the solubilization and degradation of phenanthrene in various environmental matrices. Klebsiella sp. KOD36 produced a mono-rhamnolipids biosurfactant with a low critical micelle concentration (CMC) value. The biosurfactant was stable under extreme conditions (60 °C, pH 10 and 10% salinity) and could lower surface tension by 30% and maintained an emulsification index of > 40%. The emulsion index was also higher (17-43%) in the presence of petroleum hydrocarbons compared to synthetic surfactant Triton X-100. Investigation on phenanthrene degradation in three different environmental matrices (aqueous, soil-slurry and soil) confirmed that the biosurfactant enhanced the solubilization and biodegradation of phenanthrene in all matrices. The high functional stability and performance of the biosurfactant under extreme conditions on phenanthrene degradation show the great potential of the biosurfactant for remediation applications under harsh environmental conditions.
本研究考察了极端条件下 Klebsiella sp. KOD36 产生的生物表面活性剂的稳定性和功效，及其在各种环境基质中增强菲溶解和降解的潜力。Klebsiella sp. KOD36 产生了一种低临界胶束浓度（CMC）值的单鼠李糖脂生物表面活性剂。该生物表面活性剂在极端条件下（60°C、pH 值 10 和 10%盐度）稳定，可将表面张力降低 30%，并保持>40%的乳化指数。与合成表面活性剂 Triton X-100 相比，在存在石油烃的情况下，乳液指数也更高（17-43%）。在三种不同环境基质（水相、土壤泥浆和土壤）中对菲降解的研究证实，生物表面活性剂增强了菲在所有基质中的溶解和生物降解。生物表面活性剂在极端条件下对菲降解的高功能稳定性和性能表明，生物表面活性剂在恶劣环境条件下修复应用中具有巨大潜力。
