年龄依赖性或脂质体诱导的磷脂组成变化对大鼠肝微粒体和质膜中鞘磷脂生物合成的影响。

Effects of age-dependent or liposome-induced alterations in the phospholipid composition on sphingomyelin biosynthesis in rat liver microsomal and plasma membranes.

Petkova D H, Nikolova M N, Kochlukova S E, Koumanov K S

Central Laboratory of Biophysics, Bulgarian Academy of Sciences, Sofia.

Int J Biochem. 1991;23(7-8):689-93. doi: 10.1016/0020-711x(91)90039-p.

Abstract

The effects of age-dependent or liposome-induced alterations in the phospholipid composition of rat liver plasma and microsomal membranes on the phosphatidylethanolamine:ceramide-phosphoethanolamine (PE:Cer-PEt) and phosphatidylcholine:ceramide-phosphocholine (PC:Cer-PCh) transferase activities were studied. 2. In all cases under study the PE:Cer-PEt transferase activity was found to be several times higher than that of PC:Cer-PCh transferase in both plasma and microsomal rat liver membranes. 3. The presence of phosphatidylethanolamine (PE) and phosphatidylglycerol (PG) in plasma membranes was observed to enhance the PE:Cer-PEt transferase activity, while phosphatidylserine (PS) inhibited it.

摘要

研究了年龄依赖性或脂质体诱导的大鼠肝脏血浆和微粒体膜磷脂组成变化对磷脂酰乙醇胺：神经酰胺 - 磷酸乙醇胺（PE:Cer-PEt）和磷脂酰胆碱：神经酰胺 - 磷酸胆碱（PC:Cer-PCh）转移酶活性的影响。2. 在所有研究的情况下，发现大鼠肝脏血浆和微粒体膜中PE:Cer-PEt转移酶活性比PC:Cer-PCh转移酶活性高几倍。3. 观察到质膜中磷脂酰乙醇胺（PE）和磷脂酰甘油（PG）的存在增强了PE:Cer-PEt转移酶活性，而磷脂酰丝氨酸（PS）则抑制了该活性。