Peng Y Y, Ye J N
Department of Chemistry, Quanzhou Normal University, Quanzhou, Fujian, 362000, China.
Fitoterapia. 2006 Apr;77(3):171-8. doi: 10.1016/j.fitote.2006.01.004. Epub 2006 Feb 28.
Capillary electrophoresis with electrochemical detection (CE-ED) was employed to analyze isoflavones in red clover (Trifolium pratense). The effects of potential of working electrode, pH and concentration of running buffer, separation voltage and injection time on CE-ED were investigated. Operated in a wall-jet configuration, a 300 microm diameter carbon-disk electrode was used as the working electrode, which exhibits a good response at +0.85 V (versus saturated calomel electrode) for the analytes. The analytes could be separated in a 50 mmol/l borate buffer (pH 9.5) within 25 min. The response was linear over three orders of magnitude with detection limit (S/N=3) ranging from 2x10(-5) mg/ml to 5x10(-5) mg/ml for the analytes. This method has been used for the determination of daidzein, genistein and biochanin A in red clover with satisfactory results.
采用毛细管电泳-电化学检测法（CE-ED）分析红三叶草（Trifolium pratense）中的异黄酮。研究了工作电极电位、运行缓冲液的pH值和浓度、分离电压以及进样时间对CE-ED的影响。在壁喷配置下操作，使用直径为300微米的碳盘电极作为工作电极，该电极在+0.85 V（相对于饱和甘汞电极）时对分析物表现出良好的响应。分析物可在50 mmol/l硼酸盐缓冲液（pH 9.5）中于25分钟内分离。响应在三个数量级上呈线性，分析物的检测限（S/N=3）范围为2×10⁻⁵mg/ml至5×10⁻⁵mg/ml。该方法已用于测定红三叶草中的大豆苷元、染料木黄酮和鹰嘴豆芽素A，结果令人满意。
