Shigenaga M K, Ames B N
Department of Molecular and Cell Biology, University of California, Berkeley 94720.
Free Radic Biol Med. 1991;10(3-4):211-6. doi: 10.1016/0891-5849(91)90078-h.
HPLC with electrochemical detection (HPLC-EC) is a highly sensitive and a selective method for detecting 8-hydroxy-2'-deoxyguanosine (oh8dG), a biomarker of oxidative DNA damage that is formed from hydroxyl radical attack of guanine residues in DNA. We propose that the noninvasive measurement of oh8dG in urine can be used to estimate in vivo oxidative damage. Application of this assay to urine samples obtained from rats of different ages and various species provide examples of the utility of this assay. The measurement of steady-state levels of oh8dG in DNA combined with the urinary excretion rates of oh8dG and oh8Gua, offer a powerful approach for estimating oxidative DNA damage and its repair. This method will be useful for studies designed to investigate the relationship of oxidative stress in DNA damage and the role of this damage in aging and cancer.
高效液相色谱电化学检测法（HPLC-EC）是一种高灵敏度且具选择性的方法，用于检测8-羟基-2'-脱氧鸟苷（oh8dG），它是一种氧化DNA损伤的生物标志物，由DNA中鸟嘌呤残基受到羟基自由基攻击而形成。我们提出，尿液中oh8dG的非侵入性测量可用于估计体内氧化损伤。将该检测方法应用于从不同年龄和各种物种的大鼠获得的尿液样本，提供了该检测方法实用性的实例。测量DNA中oh8dG的稳态水平，结合oh8dG和oh8Gua的尿排泄率，为估计氧化DNA损伤及其修复提供了一种有力的方法。该方法将有助于旨在研究DNA损伤中氧化应激的关系以及这种损伤在衰老和癌症中的作用的研究。
