根癌土壤杆菌ADP - 葡萄糖焦磷酸化酶的初步晶体学分析。
Preliminary crystallographic analysis of ADP-glucose pyrophosphorylase from Agrobacterium tumefaciens.
作者信息
Cupp-Vickery Jill R, Igarashi Robert Y, Meyer Christopher R
机构信息
Department of Physiology and Biophysics, University of California, Irvine, CA 92697, USA.
出版信息
Acta Crystallogr Sect F Struct Biol Cryst Commun. 2005 Mar 1;61(Pt 3):266-8. doi: 10.1107/S1744309105002265. Epub 2005 Feb 8.
ADP-glucose pyrophosphorylase catalyzes the conversion of glucose-1-phosphate and ATP to ADP-glucose and pyrophosphate, a key regulated step in both bacterial glycogen and plant starch biosynthesis. Crystals of ADP-glucose pyrophosphorylase from Agrobacterium tumefaciens (420 amino acids, 47 kDa) have been obtained by the sitting-drop vapor-diffusion method using lithium sulfate as a precipitant. A complete native X-ray diffraction data set was collected to a resolution of 2.0 A from a single crystal at 100 K. The crystals belong to space group I222, with unit-cell parameters a = 92.03, b = 141.251, c = 423.64 A. To solve the phase problem, a complete anomalous data set was collected from a selenomethionyl derivative. These crystals display one-fifth of the unit-cell volume of the wild-type crystals, with unit-cell parameters a = 85.38, b = 93.79, c = 140.29 A and space group I222.
ADP - 葡萄糖焦磷酸化酶催化葡萄糖 - 1 - 磷酸和ATP转化为ADP - 葡萄糖和焦磷酸，这是细菌糖原和植物淀粉生物合成中一个关键的调控步骤。通过坐滴气相扩散法，以硫酸锂作为沉淀剂，获得了根癌农杆菌的ADP - 葡萄糖焦磷酸化酶（420个氨基酸，47 kDa）的晶体。在100 K下从单个晶体收集了完整的天然X射线衍射数据集，分辨率达到2.0 Å。这些晶体属于空间群I222，晶胞参数为a = 92.03，b = 141.251，c = 423.64 Å。为了解决相位问题，从硒代甲硫氨酸衍生物收集了完整的反常数据集。这些晶体的晶胞体积是野生型晶体的五分之一，晶胞参数为a = 85.38，b = 93.79，c = 140.29 Å，空间群为I222。
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