2-脱氧-scyllo-肌醇合酶的结晶及X射线分析，2-脱氧链霉胺氨基糖苷类抗生素生物合成中的关键酶。
Crystallization and X-ray analysis of 2-deoxy-scyllo-inosose synthase, the key enzyme in the biosynthesis of 2-deoxystreptamine-containing aminoglycoside antibiotics.
作者信息
Nango Eriko, Kumasaka Takashi, Sato Takao, Tanaka Nobuo, Kakinuma Katsumi, Eguchi Tadashi
机构信息
Department of Chemistry, Tokyo Institute of Technology, O-okayama, Tokyo 152-8551, Japan.
出版信息
Acta Crystallogr Sect F Struct Biol Cryst Commun. 2005 Jul 1;61(Pt 7):709-11. doi: 10.1107/S1744309105018841. Epub 2005 Jun 30.
Abstract
A recombinant 2-deoxy-scyllo-inosose synthase from Bacillus circulans has been crystallized at 277 K using PEG 4000 as precipitant. The diffraction pattern of the crystal extends to 2.30 A resolution at 100 K using synchrotron radiation at the Photon Factory. The crystals are monoclinic and belong to space group P2(1), with unit-cell parameters a = 80.5, b = 70.4, c = 83.0 A, beta = 117.8 degrees. The presence of two molecules per asymmetric unit gives a crystal volume per protein weight (VM) of 2.89 A3 Da(-1) and a solvent constant of 57.4% by volume.
摘要
来自环状芽孢杆菌的一种重组2-脱氧-scyllo-肌醇合酶已在277 K下使用聚乙二醇4000作为沉淀剂进行了结晶。该晶体的衍射图谱在100 K下利用光子工厂的同步辐射扩展至2.30 Å分辨率。晶体为单斜晶系，属于空间群P2(1)，晶胞参数a = 80.5、b = 70.4、c = 83.0 Å，β = 117.8°。每个不对称单元存在两个分子，使得每蛋白重量的晶体体积（VM）为2.89 ų Da⁻¹，溶剂含量为57.4%（体积）。
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