萝芙木碱葡萄糖苷酶的异源表达、纯化、结晶及初步X射线分析，该植物酶专门参与萝芙木生物碱的生物合成。
Heterologous expression, purification, crystallization and preliminary X-ray analysis of raucaffricine glucosidase, a plant enzyme specifically involved in Rauvolfia alkaloid biosynthesis.
作者信息
Ruppert Martin, Panjikar Santosh, Barleben Leif, Stöckigt Joachim
机构信息
Department of Pharmaceutical Biology, Institute of Pharmacy, Johannes Gutenberg-University Mainz, Staudinger Weg 5, D-55099 Mainz, Germany.
出版信息
Acta Crystallogr Sect F Struct Biol Cryst Commun. 2006 Mar 1;62(Pt 3):257-60. doi: 10.1107/S174430910600457X. Epub 2006 Feb 24.
Abstract
Raucaffricine glucosidase (RG) is an enzyme that is specifically involved in the biosynthesis of indole alkaloids from the plant Rauvolfia serpentina. After heterologous expression in Escherichia coli cells, crystals of RG were obtained by the hanging-drop vapour-diffusion technique at 293 K with 0.3 M ammonium sulfate, 0.1 M sodium acetate pH 4.6 buffer and 11% PEG 4000 as precipitant. Crystals belong to space group I222 and diffract to 2.30 A, with unit-cell parameters a = 102.8, b = 127.3, c = 215.8 A.
摘要
萝芙木碱葡萄糖苷酶（RG）是一种专门参与从蛇根木植物中生物合成吲哚生物碱的酶。在大肠杆菌细胞中进行异源表达后，通过悬滴气相扩散技术，于293 K下，以0.3 M硫酸铵、0.1 M pH 4.6的醋酸钠缓冲液和11%聚乙二醇4000作为沉淀剂获得了RG晶体。晶体属于空间群I222，衍射分辨率为2.30 Å，晶胞参数a = 102.8、b = 127.3、c = 215.8 Å。
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