来自蛇根木的天然和硒代甲硫氨酸长春花碱合酶的结晶及初步X射线分析。
Crystallization and preliminary X-ray analysis of native and selenomethionyl vinorine synthase from Rauvolfia serpentina.
作者信息
Ma Xueyan, Koepke Juergen, Bayer Anja, Fritzsch Günter, Michel Hartmut, Stöckigt Joachim
机构信息
Department of Pharmaceutical Biology, Institute of Pharmacy, Johannes Gutenberg-University Mainz, Staudinger Weg 5, D-55099 Mainz, Germany.
出版信息
Acta Crystallogr D Biol Crystallogr. 2005 Jun;61(Pt 6):694-6. doi: 10.1107/S0907444904028756. Epub 2005 May 26.
Vinorine synthase (VS) is a central enzyme of the biosynthesis of the antiarrhythmic drug ajmaline and is a member of the BAHD superfamily of acyltransferases. So far, no three-dimensional structure with significant sequence homology with VS is known. Crystals of VS and selenomethionyl-labelled VS from the medicinal plant Rauvolfia serpentina have been obtained by the hanging-drop technique at 305 K with ammonium sulfate and PEG 400 as precipitants. VS crystals diffract to 2.8 A and belong to space group P2(1)2(1)2(1), with unit-cell parameters a = 82.3, b = 89.6, c = 136.2 A. The selenomethionyl VS crystal was nearly isomorphous with the VS crystal.
长春花宁合酶（VS）是抗心律失常药物阿吗灵生物合成的关键酶，属于BAHD酰基转移酶超家族成员。到目前为止，尚未发现与VS具有显著序列同源性的三维结构。通过悬滴法，以硫酸铵和聚乙二醇400作为沉淀剂，在305 K温度下，从药用植物蛇根木中获得了VS晶体以及硒代甲硫氨酸标记的VS晶体。VS晶体的衍射分辨率达到2.8 Å，属于空间群P2(1)2(1)2(1)，晶胞参数为a = 82.3 Å、b = 89.6 Å、c = 136.2 Å。硒代甲硫氨酸标记的VS晶体与VS晶体几乎同晶型。
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