Eddahri Fouad, Oldenhove Guillaume, Denanglaire Sébastien, Urbain Jacques, Leo Oberdan, Andris Fabienne
Laboratoire de Physiologie animale, Université Libre de Bruxelles, Gosselies, Belgium.
Eur J Immunol. 2006 Apr;36(4):855-63. doi: 10.1002/eji.200535500.
CD4+ CD25+ T reg cells are critical for peripheral tolerance and prevention of autoimmunity. Here we show that CD4+ CD25+ T reg also regulate the magnitude of humoral responses against a panel of T-dependent antigens of foreign origin during both primary and secondary immune responses. Depletion of CD4+ CD25+ T cells leads to increased antigen-specific antibody production and affinity maturation but does not affect T-independent B cell responses, suggesting that CD4+ CD25+ T reg exert a feedback mechanism on non-self antigen-specific antibody secretion by dampening the T cell help for B cell activation. Moreover, we show that CD4+ CD25+ T reg also suppress in vitro B cell immunoglobulin production by inhibiting CD4+ CD25- T cell help delivery, and that blockade of TGF-beta activity abolishes this suppression.
CD4+ CD25+ 调节性T细胞对于外周耐受和自身免疫的预防至关重要。在此我们表明，CD4+ CD25+ 调节性T细胞在初次和二次免疫应答期间也对外源性一组T细胞依赖性抗原的体液反应强度进行调节。CD4+ CD25+ T细胞的耗竭导致抗原特异性抗体产生增加和亲和力成熟，但不影响T细胞非依赖性B细胞反应，这表明CD4+ CD25+ 调节性T细胞通过减弱T细胞对B细胞活化的辅助作用，对非自身抗原特异性抗体分泌发挥反馈机制。此外，我们表明，CD4+ CD25+ 调节性T细胞还通过抑制CD4+ CD25- T细胞辅助作用传递在体外抑制B细胞免疫球蛋白产生，并且TGF-β活性的阻断消除了这种抑制作用。
