Calpe-Berdiel Laura, Escolà-Gil Joan Carles, Blanco-Vaca Francisco
Institut de Recerca, Hospital de la Santa Creu i Sant Pau, Barcelona, Spain.
Br J Nutr. 2006 Mar;95(3):618-22. doi: 10.1079/bjn20051659.
An increased activity of ATP-binding cassette transporter (ABC) A1 has been proposed as a mechanism underlying the hypocholesterolaemic effect of phytosterols. In the present study, ABCA1-deficient mice (ABCA1-/- mice) were used to examine the involvement of the ABCA1 in the reduction of intestinal cholesterol absorption in response to a phytosterol-enriched diet. A decrease in intestinal cholesterol absorption of 39 and 35 % was observed after phytosterol treatment in ABCA1+/+ mice and in ABCA1-/- mice, respectively. No statistically significant changes in plasma lipoprotein profile or in intestinal ABCG5, ABCG8 and Niemann-Pick C1-Like 1 gene expression levels were found when phytosterol-treated ABCA1-/- mice and untreated ABCA1-/- mice were compared. We conclude that phytosterol inhibition of cholesterol absorption in mice is independent of ABCA1.
有人提出，ATP结合盒转运蛋白（ABC）A1活性增加是植物甾醇降胆固醇作用的潜在机制。在本研究中，使用ABCA1基因缺陷小鼠（ABCA1-/-小鼠）来研究ABCA1在富含植物甾醇饮食诱导的肠道胆固醇吸收减少中的作用。植物甾醇处理后，ABCA1+/+小鼠和ABCA1-/-小鼠的肠道胆固醇吸收分别降低了39%和35%。比较植物甾醇处理的ABCA1-/-小鼠和未处理的ABCA1-/-小鼠时，未发现血浆脂蛋白谱或肠道ABCG5、ABCG8和尼曼-皮克C1样1基因表达水平有统计学显著变化。我们得出结论，植物甾醇对小鼠胆固醇吸收的抑制作用独立于ABCA1。
