Liang Huan Ling, Wong-Riley Margaret T T
Department of Cell Biology, Neurobiology and Anatomy, Medical College of Wisconsin, Milwaukee, Wisconsin 53226, USA.
Neuroreport. 2006 Mar 20;17(4):401-5. doi: 10.1097/01.wnr.0000204980.98876.11.
Nuclear respiratory factor-1 and nuclear respiratory factor-2 activate the transcription of several respiratory chain enzymes and are prime candidates for bigenomic coordinated regulation of cytochrome oxidase subunit genes from the two genomes. Peroxisome proliferator-activated receptor gamma coactivator 1 is a proposed coactivator of nuclear respiratory factor-1 and nuclear respiratory factor-2-dependent transcription, but its significance and function in neurons are unknown. Our current study indicates that nuclear respiratory factor-1, nuclear respiratory factor-2, and peroxisome proliferator-activated receptor gamma coactivator-1 are expressed in rat visual cortical neurons, and that neuronal activity directly regulates the protein and mRNA expressions of these factors after functional inactivation in vivo and in vitro. Changes in peroxisome proliferator-activated receptor gamma coactivator-1 preceded those of nuclear respiratory factor-1 and nuclear respiratory factor-2, suggesting that it may be the prime sensor of neuronal activity and its energy demand.
核呼吸因子-1和核呼吸因子-2可激活多种呼吸链酶的转录，是对来自两个基因组的细胞色素氧化酶亚基基因进行双基因组协同调控的主要候选因子。过氧化物酶体增殖物激活受体γ共激活因子1被认为是核呼吸因子-1和核呼吸因子-2依赖性转录的共激活因子，但其在神经元中的意义和功能尚不清楚。我们目前的研究表明，核呼吸因子-1、核呼吸因子-2和过氧化物酶体增殖物激活受体γ共激活因子-1在大鼠视皮层神经元中表达，并且在体内和体外功能失活后，神经元活动直接调节这些因子的蛋白质和mRNA表达。过氧化物酶体增殖物激活受体γ共激活因子-1的变化先于核呼吸因子-1和核呼吸因子-2的变化，这表明它可能是神经元活动及其能量需求的主要传感器。
