实验性自身免疫性神经炎大鼠坐骨神经中核因子-κB的免疫定位及激活
Immunolocalization and activation of nuclear factor-kappaB in the sciatic nerves of rats with experimental autoimmune neuritis.
作者信息
Laurà M, Mazzeo A, Aguennouz M, Santoro M, Catania M A, Migliorato A, Calapai G, Vita G
机构信息
Department of Neuroscience, Psichiatry and Anaesthesiology, University of Messina, Italy.
出版信息
J Neuroimmunol. 2006 May;174(1-2):32-8. doi: 10.1016/j.jneuroim.2006.01.004. Epub 2006 Mar 6.
Recent data support an important role played by nuclear factor kappa B (NF-kappaB) in peripheral neuropathies. We investigated expression and activation of NF-kappaB in experimental autoimmune neuritis (EAN) in rat sciatic nerves removed after 7, 14 and 21 days after immunization. Immunoreactivity for the activated form of NF-kappaB was found in the nuclei of T cells and macrophages at days 14 and 21, and also in the nuclei of few Schwann cells and of vascular endothelial cells at all time points, especially during the peak stage. Western blot showed a single band corresponding to 65 kDa in all EAN animals. NF-kappaB DNA-binding activity was revealed by electrophoretic mobility shift assay. Our results support NF-kappaB activation in EAN during the induction stage as well as in the disease remission.
近期数据支持核因子κB（NF-κB）在周围神经病变中发挥重要作用。我们研究了免疫后7天、14天和21天取出的大鼠坐骨神经中NF-κB在实验性自身免疫性神经炎（EAN）中的表达和激活情况。在第14天和21天，在T细胞和巨噬细胞核中发现了NF-κB活化形式的免疫反应性，并且在所有时间点，尤其是在高峰期，少数施万细胞和血管内皮细胞核中也发现了这种反应性。蛋白质印迹法显示所有EAN动物中均有一条对应于65 kDa的单条带。通过电泳迁移率变动分析揭示了NF-κB的DNA结合活性。我们的结果支持EAN在诱导期以及疾病缓解期NF-κB的激活。
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