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阿米洛利与DNA双链体中AP位点对面的胸腺嘧啶碱基具有强选择性结合：同时与DNA磷酸骨架结合。

Strong and selective binding of amiloride to thymine base opposite AP sites in DNA duplexes: simultaneous binding to DNA phosphate backbone.

作者信息

Zhao Chunxia, Dai Qing, Seino Takehiro, Cui Ying-Yu, Nishizawa Seiichi, Teramae Norio

机构信息

Department of Chemistry, Graduate School of Science, Tohoku University, Aoba-ku, Sendai 980-8578, Japan.

出版信息

Chem Commun (Camb). 2006 Mar 21(11):1185-7. doi: 10.1039/b516575j. Epub 2006 Jan 30.

DOI:10.1039/b516575j
PMID:16518485
Abstract

Amiloride (N-amidino-3,5-diamino-6-chloro-pyrazinecarboxamide hydrochloride) has two sets of hydrogen-bond forming sites suitable for target nucleotides and the phosphodiester DNA backbone by which a thymine base opposite an abasic site in DNA duplexes can be recognized with high selectivity and affinity, and it is applicable to the fluorescence detection of thymidine-related SNPs (single-nucleotide polymorphisms) of PCR amplification products.

摘要

氨氯吡脒（盐酸N-脒基-3,5-二氨基-6-氯吡嗪甲酰胺）有两组适合与靶核苷酸和磷酸二酯DNA主链形成氢键的位点，通过这些位点，DNA双链体中无碱基位点对面的胸腺嘧啶碱基能够以高选择性和亲和力被识别，并且它适用于PCR扩增产物中胸腺嘧啶核苷相关单核苷酸多态性（SNP）的荧光检测。

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Chem Commun (Camb). 2006 Mar 21(11):1185-7. doi: 10.1039/b516575j. Epub 2006 Jan 30.
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