Fukushima Michihiro, Han Kyu-Ho, Taneichi Yae, Iijima Setsuko, Shimada Ken-ichiro, Saitoh Katsuichi, Oda Yuji, Chiji Hideyuki, Sekikawa Mitsuo
Department of Animal Science, Obihiro University of Agriculture and Veterinary Medicine, Obihiro, Hokkaido 080-8555, Japan.
J Nutr Sci Vitaminol (Tokyo). 2005 Dec;51(6):453-9. doi: 10.3177/jnsv.51.453.
We examined the effects of Amylomyces rouxii, which is a mold found in some fermented foods in Indonesia, on serum cholesterol and hepatic LDL receptor mRNA in rats. Rats were fed a 0.5% cholesterol-enriched diet with (A. rouxii group) or without (control group) 30 g/kg A. rouxii for 4 wk. There were no significant differences in the body weight, food intake or liver weight among the groups. However, the weight of the cecum in the A. rouxii-fed group was significantly higher than that in the control group. The cecal pH in the A. rouxii-fed group was significantly lower than that in the control group. Cecal acetic acid, propionic acid and total SCFA concentrations in the A. rouxii-fed group were significantly higher than those in the control group. The serum total cholesterol and VLDL+intermediate density lipoprotein (IDL)+LDL-cholesterol concentrations in the control group were significantly higher than those in the A. rouxii-fed group at the end of the 4-wk feeding period. There were no significant differences in the HDL-cholesterol or triglyceride concentrations between the groups. The hepatic LDL receptor and cholesterol 7alpha-hydroxylase mRNA levels in the A. rouxii-fed group were significantly higher than those in the control group. The results of this study demonstrate that feeding of A. rouxii lowers the serum total cholesterol level by enhancement of the cecal SCFA concentration and the hepatic LDL receptor mRNA.
我们研究了鲁氏毛霉（一种在印度尼西亚一些发酵食品中发现的霉菌）对大鼠血清胆固醇和肝脏低密度脂蛋白受体mRNA的影响。给大鼠喂食含0.5%胆固醇的饲料，持续4周，其中一组添加30 g/kg鲁氏毛霉（鲁氏毛霉组），另一组不添加（对照组）。各组之间在体重、食物摄入量或肝脏重量方面没有显著差异。然而，喂食鲁氏毛霉组的盲肠重量显著高于对照组。喂食鲁氏毛霉组的盲肠pH值显著低于对照组。喂食鲁氏毛霉组的盲肠乙酸、丙酸和总短链脂肪酸浓度显著高于对照组。在4周喂食期结束时，对照组的血清总胆固醇和极低密度脂蛋白+中间密度脂蛋白（IDL）+低密度脂蛋白胆固醇浓度显著高于喂食鲁氏毛霉组。各组之间的高密度脂蛋白胆固醇或甘油三酯浓度没有显著差异。喂食鲁氏毛霉组的肝脏低密度脂蛋白受体和胆固醇7α-羟化酶mRNA水平显著高于对照组。本研究结果表明，喂食鲁氏毛霉可通过提高盲肠短链脂肪酸浓度和肝脏低密度脂蛋白受体mRNA来降低血清总胆固醇水平。
