Terekhova L P, Novozhenov M Iu, Tolstykh I V, Brazhnikova M G
Antibiot Khimioter. 1991 Mar;36(3):15-7.
Methodical approaches to detecting cultures producing streptothricins at the early stages of screening new antibiotics were developed. The approaches are based on chromatographic and electrophoretic mobility of streptothricins and the products of their hydrolysis in the extracts from agar cultures of actinomycetes. Application of the method for screening new antibiotics is illustrated with an example. Nine strains of actinomyces with broad antibacterial spectra isolated from soil samples were studied and 6 of them belonging to 4 species were shown to produce streptothricins in agar cultures. The new streptothricin-producing culture S. roseolilacinus was isolated.
开发了在筛选新抗生素的早期阶段检测产生链丝菌素的培养物的系统方法。这些方法基于链丝菌素及其在放线菌琼脂培养物提取物中的水解产物的色谱和电泳迁移率。通过一个例子说明了该方法在筛选新抗生素中的应用。对从土壤样品中分离出的9株具有广泛抗菌谱的放线菌菌株进行了研究,结果表明其中6株属于4个物种,在琼脂培养物中产生链丝菌素。分离出了新的产生链丝菌素的培养物——玫瑰紫链霉菌。