[缺氧对培养内皮细胞中磷酸肌醇代谢及腺苷酸环化酶系统的影响]

[Effects of hypoxia on phosphoinositide turnover and adenylate cyclase system in cultured endothelial cells].

作者信息

Buravkova L B, Mirzapoiazova T Iu, Grigorian G Iu, Tkachuk V A

出版信息

Biull Eksp Biol Med. 1991 May;111(5):464-6.

Abstract

By studying the effects of oxygen deficiency upon signal-transducing system it has been shown that in long hypobaric hypoxia activates PI-turnover in cultured human endothelial cells. The sensitivity of cells to histamine was decreased as well as the adenylate cyclase activity in membranes of this cells. The amount of beta-adrenoreceptors was not influenced significantly. Incubation of endothelial cells with histamine (10(-5) M) and phorbol ester (10(-9) M) = activator of protein kinase C within 1-2 h resulted in desensitization of cellular responses which can be seen not only as a disappearance of histamine-induced activation of PI-turnover but also as a decrease of beta-adrenoreceptor amount and adenylate cyclase activity. It seems that hypoxia may change the action of Ca-mobilizing hormones on PI-turnover and suppress adenylate cyclase in human endothelial cells. However the effects of hypoxia on signal-transducing systems in this cells are developed slower than those of Ca-mobilizing hormones.

摘要

通过研究缺氧对信号转导系统的影响，结果表明，在长时间低压缺氧条件下，培养的人内皮细胞中的磷脂酰肌醇（PI）代谢被激活。细胞对组胺的敏感性降低，同时该细胞细胞膜中的腺苷酸环化酶活性也降低。β-肾上腺素能受体的数量未受到显著影响。将内皮细胞与组胺（10⁻⁵ M）和佛波酯（10⁻⁹ M，蛋白激酶C的激活剂）一起孵育1 - 2小时，会导致细胞反应脱敏，这不仅表现为组胺诱导的PI代谢激活消失，还表现为β-肾上腺素能受体数量和腺苷酸环化酶活性降低。似乎缺氧可能改变钙动员激素对PI代谢的作用，并抑制人内皮细胞中的腺苷酸环化酶。然而，缺氧对该细胞信号转导系统的影响比钙动员激素的影响发展得更慢。