克氏锥虫而非布氏锥虫无法在巨噬细胞杂交瘤细胞系中诱导化学发光信号。
Trypanosoma cruzi but not Trypanosoma brucei fails to induce a chemiluminescent signal in a macrophage hybridoma cell line.
作者信息
Vray B, De Baetselier P, Ouaissi A, Carlier Y
机构信息
Laboratoire de Parasitologie Expérimentale, Faculté des Sciences, Université Libre de Bruxelles, Belgium.
出版信息
Infect Immun. 1991 Sep;59(9):3303-8. doi: 10.1128/iai.59.9.3303-3308.1991.
Abstract
Macrophage-Trypanosoma cruzi interactions were studied by using a newly generated macrophage hybridoma cell line (2C11-12) that was selected for its capacity to produce high levels of reactive oxygen intermediates. This cell line was found to be a suitable host cell for T. cruzi, and intracellular parasitic development could be inhibited by activation with gamma interferon. When exposed to opsonized Trypanosoma brucei, Micrococcus lysodeikticus, or Legionella pneumophila, the activated macrophage cell line produces a high chemiluminescent signal, indicating the release of reactive oxygen intermediates. Alternatively, when opsonized T. cruzi was added to these activated macrophages, this parasite failed to stimulate a chemiluminescent response, suggesting an impairment in the triggering of the respiratory burst.
摘要
通过使用新产生的巨噬细胞杂交瘤细胞系（2C11-12）研究巨噬细胞与克氏锥虫的相互作用，该细胞系因其产生高水平活性氧中间体的能力而被筛选出来。发现该细胞系是克氏锥虫合适的宿主细胞，γ干扰素激活可抑制细胞内寄生虫的发育。当暴露于调理过的布氏锥虫、溶壁微球菌或嗜肺军团菌时，活化的巨噬细胞系会产生高化学发光信号，表明活性氧中间体的释放。或者，当将调理过的克氏锥虫添加到这些活化的巨噬细胞中时，这种寄生虫未能刺激化学发光反应，表明呼吸爆发的触发受到损害。
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