Zhang Shu-Hong, Yao Ji-Hua, Song Huai-Dong, Wang Lu, Xue Jing-Lun
State Key Laboratory of Genetic Engineering, Institute of Genetics, School of Life Sciences, Fudan University, Shanghai, China.
Int J Dev Biol. 2006;50(4):399-403. doi: 10.1387/ijdb.052029sz.
We have identified translation elongation factor 2 (EF-2) in zebrafish (GenBank Accession No. AAQ91234). Analysis of the DNA sequence of zebrafish EF-2 shows that the 2826 bp cDNA spans an open reading frame between nucleotide 55 to 2631 and encodes a protein of 858 amino acids. Zebrafish EF-2 protein shares 92%, 93%, 93% and 92% identity with the corresponding amino acid sequence in human, mouse, Chinese hamster and Gallus EF-2, respectively. Whole-mount in situ hybridization showed that zebrafish EF-2 was a developmentally regulated gene and might play important roles during the early development of zebrafish embryos. Therefore, we further studied the function of EF-2 during early embryogenesis. Using morpholino antisense oligo knockdown assays, anti-MO injected embryos were found to display abnormal development. The yolk balls were larger than normal and the melanophores spreading on their bodies became fewer. Furthermore, their tails were incurvate and their lenses were much smaller than those of the normal embryos. However the EF-2 overexpression data showed that extra EF-2 protein had no obvious effect on zebrafish embryonic development.
我们已在斑马鱼中鉴定出翻译延伸因子2（EF-2）（GenBank登录号：AAQ91234）。对斑马鱼EF-2的DNA序列分析表明，2826 bp的cDNA跨越了55至2631核苷酸之间的一个开放阅读框，编码一个858个氨基酸的蛋白质。斑马鱼EF-2蛋白与人、小鼠、中国仓鼠和鸡的EF-2相应氨基酸序列的同一性分别为92%、93%、93%和92%。全胚胎原位杂交显示，斑马鱼EF-2是一个受发育调控的基因，可能在斑马鱼胚胎的早期发育过程中发挥重要作用。因此，我们进一步研究了EF-2在早期胚胎发生过程中的功能。使用吗啉代反义寡核苷酸敲低试验，发现注射抗吗啉代的胚胎显示出发育异常。卵黄球比正常的大，身体上扩散的黑素细胞变少。此外，它们的尾巴弯曲，晶状体比正常胚胎的小得多。然而，EF-2过表达数据显示，额外的EF-2蛋白对斑马鱼胚胎发育没有明显影响。
