Tough D F, Chow D A
Department of Immunology, University of Manitoba, Winnipeg, Canada.
Nat Immun Cell Growth Regul. 1991;10(2):83-93.
An increasing body of evidence supports a role for natural antitumor antibodies acting against tumors in vivo. However, a role for natural antibodies (NAb) in tumor surveillance would imply that sensitivity to NAb should increase following events associated with cellular transformation. To test this prediction we examined by flow cytometry the effect on serum NAb binding of v-H-ras expression and integration in 10T1/2. The co-introduction of v-H-ras and the neomycin resistance (neor) gene into 10T1/2 followed by G418 selection resulted in a marked and heterogeneous increase in NAb binding. Clonal analysis of this population demonstrated that the increased NAb binding was associated with tumorigenic conversion and ras-p21 expression. The results provide the first evidence for a NAb-susceptible phase of ras-induced transformation.
越来越多的证据支持天然抗肿瘤抗体在体内对肿瘤发挥作用。然而，天然抗体（NAb）在肿瘤监测中的作用意味着，在与细胞转化相关的事件发生后，对NAb的敏感性应该会增加。为了验证这一预测，我们通过流式细胞术检测了v-H-ras在10T1/2细胞中的表达和整合对血清NAb结合的影响。将v-H-ras和新霉素抗性（neor）基因共同导入10T1/2细胞，随后进行G418筛选，导致NAb结合显著且异质性增加。对该群体的克隆分析表明，NAb结合增加与致瘤性转化和ras-p21表达有关。这些结果为ras诱导的转化存在NAb敏感阶段提供了首个证据。
