Duex J M, Cordin X, Péchinot A, Siebor E, Kazmierczak A
Laboratoire de Bactériologie-Hygiène, Centre Hospitalier, Universitaire de Dijon, Hôpital du Bocage.
Pathol Biol (Paris). 1991 May;39(5):374-8.
The combination of piperacillin and tazobactam was studied in a microtiter checkerboard assay against 160 strains of Enterobacteriaceae selected for their resistance pattern to amoxicillin, ticarcillin, cephalothin and cefotaxime. Mean FIC indices were respectively 0.02, 0.17 and 0.01 for RRSS strains of E. coli, K. pneumoniae and P. mirabilis. Mean FIC indices were 0.03 for RRRI/R strains of K. pneumoniae producing a broad spectrum beta-lactamase, but the synergistic effect was out of therapeutic range for 16 strains of 20. Mean FIC indices were 0.53, 0.90, 0.55, 0.10 and 0.21 respectively against RSRS strains of E. coli, E. cloacae, S. marcescens, M. morganii and P. vulgaris. Mean FIC indices were 0.02, 0.07, 0.02 and 0.01 against RRRS/R strains of E. coli, E. cloacae, S. marcescens and M. morganii but they were in a therapeutical range (piperacillin less than or equal to 8 mg/l and tazobactam less than or equal to 4 mg/l) respectively for 7, 0, 4 and 10 strains of 10 in each species. Tazobactam restored the antibacterial activity of piperacillin against enterobacterial strains mainly producing a penicillinase or a cephalosporinase for M. morganii and P. vulgaris.
采用微量滴定棋盘法，研究了哌拉西林与他唑巴坦联合对160株肠杆菌科细菌的抗菌活性，这些菌株是根据它们对阿莫西林、替卡西林、头孢噻吩和头孢噻肟的耐药模式挑选出来的。大肠杆菌、肺炎克雷伯菌和奇异变形杆菌RRSS菌株的平均FIC指数分别为0.02、0.17和0.01。产生广谱β-内酰胺酶的肺炎克雷伯菌RRRI/R菌株的平均FIC指数为0.03，但20株中有16株的协同效应超出治疗范围。针对大肠杆菌、阴沟肠杆菌、粘质沙雷氏菌、摩根氏菌和普通变形杆菌的RSRS菌株，平均FIC指数分别为0.53、0.90、0.55、0.10和0.21。针对大肠杆菌、阴沟肠杆菌、粘质沙雷氏菌和摩根氏菌的RRRS/R菌株，平均FIC指数分别为0.02、0.07、0.02和0.01，但在每个菌种的10株菌株中，分别有7、0、4和10株处于治疗范围内（哌拉西林小于或等于8mg/L，他唑巴坦小于或等于4mg/L）。他唑巴坦恢复了哌拉西林对主要产生青霉素酶或头孢菌素酶的摩根氏菌和普通变形杆菌肠杆菌菌株的抗菌活性。
