Patel Amrutlal K, Tikoo Suresh K
Vectored Vaccine Program, Vaccine and Infectious Disease Organization, University of Saskatchewan, Saskatoon, SK S7N 5E3, Canada.
J Gen Virol. 2006 Apr;87(Pt 4):817-821. doi: 10.1099/vir.0.81305-0.
Human cells do not normally support productive bovine adenovirus type 3 (BAdV-3) infection. Here, the outcome of BAdV-3 infection of both 293 cells and 293 cells modified to constitutively express the simian virus 40 (SV-40) T antigen (293T cells) was studied. Whereas BAdV-3 could efficiently infect 293 cells, there was a block in virus DNA replication, late-gene expression and virus production. In contrast, replication and efficient virus production could be detected in 293T cells infected with BAdV-3 or transfected with a replication-competent genomic BAdV-3 clone (pFBAV304). Early-phase gene expression was detected readily in both BAdV-3-infected 293 and 293T cells. However, the progression to efficient viral DNA synthesis and late-phase protein synthesis occurred only in 293T cells. Electron microscopy and virus growth kinetics demonstrated the formation of progeny virus in 293T cells. The SV-40 T antigens act to overcome a barrier in BAdV-3 DNA replication in 293 cells.
人类细胞通常不支持牛3型腺病毒（BAdV - 3）的有效感染。在此，研究了BAdV - 3对293细胞和经修饰组成性表达猿猴病毒40（SV - 40）T抗原的293细胞（293T细胞）的感染结果。虽然BAdV - 3能够有效感染293细胞，但在病毒DNA复制、晚期基因表达和病毒产生方面存在障碍。相比之下，在用BAdV - 3感染或用具有复制能力的基因组BAdV - 3克隆（pFBAV304）转染的293T细胞中可检测到复制和高效的病毒产生。在BAdV - 3感染的293细胞和293T细胞中均能轻易检测到早期基因表达。然而，只有在293T细胞中才会进展到有效的病毒DNA合成和晚期蛋白质合成。电子显微镜和病毒生长动力学证明了293T细胞中产生子代病毒。SV - 40 T抗原的作用是克服293细胞中BAdV - 3 DNA复制的障碍。
