丙型肝炎病毒相关的内部核糖体进入位点存在于小核糖核酸病毒科的多个属中。

Hepatitis C virus-related internal ribosome entry sites are found in multiple genera of the family Picornaviridae.

作者信息

Chard Louisa S, Bordeleau Marie-Eve, Pelletier Jerry, Tanaka Junichi, Belsham Graham J

机构信息

BBSRC, Institute for Animal Health, Pirbright, Woking, Surrey GU24 0NF, UK.

Department of Biochemistry, McIntyre Medical Sciences Building, Montreal, QC H3G 1Y6, Canada.

出版信息

J Gen Virol. 2006 Apr;87(Pt 4):927-936. doi: 10.1099/vir.0.81546-0.

DOI:10.1099/vir.0.81546-0

PMID:16528042

Abstract

The internal ribosome entry site (IRES) elements from porcine enterovirus 8 and simian virus 2, two members of a proposed new genus within the family Picornaviridae, were characterized. These IRES elements, in common with the porcine teschovirus 1 IRES, were found to be related functionally and structurally to the IRES element from Hepatitis C virus, a member of the family Flaviviridae. Partial secondary structure predictions were derived and functional assays demonstrated that these IRES elements continued to be active when eIF4G was cleaved and when the activity of eIF4A was blocked.

摘要

对猪肠道病毒8和猿猴病毒2（这两种病毒是小RNA病毒科中一个拟议新属的成员）的内部核糖体进入位点（IRES）元件进行了表征。发现这些IRES元件与猪捷申病毒1的IRES元件一样，在功能和结构上与黄病毒科成员丙型肝炎病毒的IRES元件相关。推导了部分二级结构预测结果，功能分析表明，当eIF4G被切割以及eIF4A的活性被阻断时，这些IRES元件仍具有活性。