通过乳胶凝集试验检测粪便中的猪轮状病毒。
Detection of porcine rotavirus in stools by a latex agglutination test.
作者信息
Sanekata T, Kishimoto E, Sato K, Honma H, Otsuki K, Tsubokura M
机构信息
Department of Microbiology, Faculty of Agriculture, Tottori University, Japan.
出版信息
Vet Microbiol. 1991 May;27(3-4):245-51. doi: 10.1016/0378-1135(91)90151-5.
We developed a simple agglutination test for the detection of porcine rotavirus in stools from pigs with diarrhea. The virus was detected with high sensitivity and specificity by a slide agglutination test using latex particles coated with antibody against the porcine rotavirus strain OSU (LA-antiOSU). The agglutination of LA-antiOSU with OSU on a glass slide was evident macroscopically within 2 min. The sensitivity of this latex agglutination (LA) test was four times higher than that of the electron microscope method. The LA test is available for the rapid diagnosis of porcine rotavirus infections.
我们开发了一种简单的凝集试验，用于检测腹泻猪粪便中的猪轮状病毒。通过使用包被有抗猪轮状病毒OSU株抗体的乳胶颗粒（LA-antiOSU）进行玻片凝集试验，以高灵敏度和特异性检测该病毒。LA-antiOSU与OSU在玻片上的凝集在2分钟内肉眼可见。这种乳胶凝集（LA）试验的灵敏度比电子显微镜法高四倍。LA试验可用于猪轮状病毒感染的快速诊断。
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