Schlueter Annette J, Glasgow Judith K
Department of Pathology, University of Iowa Carver College of Medicine, Iowa City, 52242, USA.
Cytometry A. 2006 Apr;69(4):281-90. doi: 10.1002/cyto.a.20262.
Monocyte subsets are not well defined in murine peripheral blood (PB). Monocyte-related populations could also be located in bone marrow (BM), but studies correlating monocyte populations found in these two tissues are lacking. This study simultaneously analyzed PB and BM to phenotypically define multiple monocyte-related subsets in each location.
Murine PB and BM cells were simultaneously stained for monocyte-related populations, using five-color flow cytometry. Relevant subsets were defined on the basis of Ly-6C, CD11b, and wheat germ agglutinin phenotype in addition to light-scatter characteristics. These populations were extensively characterized for the expression of other myeloid and dendritic cell markers, adhesion molecules, chemokine receptors, and growth factor receptors.
Six monocyte-related populations were defined, three each in BM and PB. No identical populations were found between the two tissues. Two BM populations and one PB population have heterogeneous expression of many markers, suggesting additional complexity among monocyte-related subsets.
The murine monocytic series comprises multiple subsets, differing between PB and BM. This study defines and extensively phenotypes six of these populations, providing preliminary information about possible developmental relationships and migratory capacities of these cells.
小鼠外周血（PB）中的单核细胞亚群尚未得到很好的定义。单核细胞相关群体也可能存在于骨髓（BM）中，但缺乏对这两种组织中发现的单核细胞群体进行相关性研究。本研究同时分析了PB和BM，以便从表型上定义每个部位的多个单核细胞相关亚群。
使用五色流式细胞术对小鼠PB和BM细胞同时进行单核细胞相关群体染色。除了光散射特征外，还根据Ly-6C、CD11b和麦胚凝集素表型定义相关亚群。对这些群体进行了广泛的表征，以检测其他髓系和树突状细胞标志物、黏附分子、趋化因子受体和生长因子受体的表达。
定义了六个单核细胞相关群体，BM和PB中各有三个。在这两个组织之间未发现相同的群体。两个BM群体和一个PB群体具有许多标志物的异质性表达，表明单核细胞相关亚群之间存在额外的复杂性。
小鼠单核细胞系列包含多个亚群，PB和BM中的亚群不同。本研究定义并广泛表征了其中六个群体的表型，提供了有关这些细胞可能的发育关系和迁移能力的初步信息。
