Yamauchi T, Sekiya K, Okuda H, Kimura S
2nd Department of Medical Biochemistry, School of Medicine, Ehime University, Japan.
Agressologie. 1991;32(2):115-20.
Insulin-stimulated 2-deoxy-D-glucose uptake by the rat soleus muscle was concluded to be closely related to Na+/H+ exchanger from the following findings; (1) Insulin stimulated Na+ uptake by rat soleus muscle, (2) Amiloride inhibited the effect of insulin on 2-deoxy-D-glucose uptake by the muscle. (3) External Na+ or Li+ was essential for the action of insulin, and Na+ could not be replaced by other monovalent cations such as K+, Rb+, and choline+. The decreases in the effect of insulin on reduction of the pH of the medium, addition of amiloride, or solution for replacement of Na(+)-Hanks buffer by choline(+)-Hanks buffer could not be explained by decrease in specific binding of insulin.
胰岛素刺激大鼠比目鱼肌摄取2-脱氧-D-葡萄糖与Na⁺/H⁺交换体密切相关；（1）胰岛素刺激大鼠比目鱼肌摄取Na⁺；（2）氨氯地平抑制胰岛素对肌肉摄取2-脱氧-D-葡萄糖的作用；（3）细胞外Na⁺或Li⁺对胰岛素的作用至关重要，且Na⁺不能被其他单价阳离子如K⁺、Rb⁺和胆碱⁺替代。胰岛素对培养基pH降低作用的减弱、添加氨氯地平或用胆碱⁺-Hanks缓冲液替代Na⁺-Hanks缓冲液，不能用胰岛素特异性结合的减少来解释。
