Fan Weijie, Yang Zhiming, Luo Jingcong, Huang Yun, Li Xiuqun, Wang Zhen
Division of Stem Cell and Tissue Engineering, State Key Laboratory of Biotherapy, West China Hospital, Sichuan University, Chengdu Sichuan 610041, P R China.
Zhongguo Xiu Fu Chong Jian Wai Ke Za Zhi. 2006 Feb;20(2):155-60.
To compare the reparative effects between the acellular small intestinal submucosa and the acellular amnion as dressings for traumatic skin defects.
Three full-thickness skin defects, which were close to the vertebral column of the pig, were created on both sides of the dorsum. The skin defects were randomly divided into three groups. In each group, the following different materials were used to cover the skin defects: the acellular amnion in Group A, the acellular small intestinal submucosa (SIS) in Group B, and the physiological saline gauze in Group C (the control group). The specimens from the skin defects were harvested for a histological evaluation and for determination of the hydroxyproline content at 10 (2 pigs), 20 (2 pigs), and 30 days (3 pigs). We observed the healing process of the wound and its healing rate, counted the inflammatory cells, vascular endothelial cells, and proliferating cells, and determined the hydroxyproline content.
The acellular amnion in Group A and the acellular SIS in Group B adhered to the wound tightly, but they did not adhere to the dressing; when the dressing was changed, the wound did not bleed. The saline gauze in Group C adhered to the wound tightly, but when the dressing was changed, the wound bled until 22 days after operation. Under the microscope, the collagen in the tissue below the epithelium was arranged more regularly and there were fewer cells concerned with inflammation in Groups A and B than in Group C at 10, 20, and 30 days after operation. At 10, 20, and 30 days after operation, the wound healing rate was greater in Groups A and B than in Group C. The number of the inflammatory cells and the proliferating cells were greater in Group C than in Groups A and B. There was a statistically significant difference (P < 0.05), At 20 and 30 days after operation, the content of hydroxyproline was greater in Group C than in Group A and B. There was a statistically significant difference (P < 0.05). However, there was no statistically significant difference between Group A and Group B in the wound healing rate, the numbers of the inflammatory cells, vascular endothelial cells and proliferating cells, and the content of hydroxyproline (P > 0.05). There was no statistically significant difference among the three groups in the number of the vascular endothelial cells.
Compared with Group C, the wound healing rate is improved, the collagen in the tissue below the epithelium is arranged more regularly, and the inflammatory cells, bleeding, and effusion are reduced in Groups A and B. The reparative effects of the acellular amnion and the acellular small intestinal submucosa as dressings on the skin defects are almost the same.
比较脱细胞小肠黏膜下层与脱细胞羊膜作为创伤性皮肤缺损敷料的修复效果。
在猪背部两侧靠近脊柱处制造三个全层皮肤缺损。将皮肤缺损随机分为三组。每组使用以下不同材料覆盖皮肤缺损：A组为脱细胞羊膜，B组为脱细胞小肠黏膜下层（SIS），C组为生理盐水纱布（对照组）。在术后10天（2头猪）、20天（2头猪）和30天（3头猪）从皮肤缺损处采集标本进行组织学评估并测定羟脯氨酸含量。观察伤口愈合过程及其愈合率，计数炎症细胞、血管内皮细胞和增殖细胞，并测定羟脯氨酸含量。
A组的脱细胞羊膜和B组的脱细胞SIS与伤口紧密粘连，但不与敷料粘连；换药时伤口不出血。C组的生理盐水纱布与伤口紧密粘连，但换药时伤口一直出血直至术后22天。显微镜下，术后10天、20天和30天时，A组和B组上皮下组织中的胶原排列更规则，炎症相关细胞比C组少。术后10天、20天和30天时，A组和B组的伤口愈合率高于C组。C组的炎症细胞和增殖细胞数量多于A组和B组。差异有统计学意义（P<0.05）。术后20天和30天时，C组的羟脯氨酸含量高于A组和B组。差异有统计学意义（P<0.05）。然而，A组和B组在伤口愈合率、炎症细胞、血管内皮细胞和增殖细胞数量以及羟脯氨酸含量方面差异无统计学意义（P>0.05）。三组间血管内皮细胞数量差异无统计学意义。
与C组相比，A组和B组伤口愈合率提高，上皮下组织中胶原排列更规则，炎症细胞、出血和渗出减少。脱细胞羊膜和脱细胞小肠黏膜下层作为皮肤缺损敷料的修复效果几乎相同。
