Zhang Chao, Jin Yan, Nie Xin, Liu Yuan, Lei Juan, Hu Dan
Department of Oral Histology and Pathology, Stomatological Collage, Fourth Military Medical University, Xi'an Shaanxi 710032, P R China.
Zhongguo Xiu Fu Chong Jian Wai Ke Za Zhi. 2006 Feb;20(2):185-8.
To observe the biocompatibility of the acellular corneal stroma materials prepared by three different methods.
Three different serial digestion methods were used to produce the acellular corneal stroma materials. The biocompatibility of the materials was investigated by the cell seeding and the materials were implanted into the rabbit corneal stroma layer.
The cells in the materials 1 and 2 were not decellularized completely. The rabbit corneal fibroblasts died on the materials 1 and 2 after the cell seeding for 3-4 days. An obvious rejection could be observed after the implantation. The cells in material 3 were decellularized completely and the collagen fibers or elastic fibers were reserved integrally, showing a typical three-dimensional network. The rabbit corneal fibroblasts could expand on the materials in vitro. No obvious rejection could be observed and the materials were gradually absorbed.
The acellular porcine cornea stroma materials prepared by trypsin-Dnase-Rnase are suitable for reconstruction of the tissue engineered cornea.
观察三种不同方法制备的脱细胞角膜基质材料的生物相容性。
采用三种不同的连续消化方法制备脱细胞角膜基质材料。通过细胞接种研究材料的生物相容性，并将材料植入兔角膜基质层。
材料1和材料2中的细胞未完全脱细胞。接种细胞3 - 4天后，兔角膜成纤维细胞在材料1和材料2上死亡。植入后可观察到明显的排斥反应。材料3中的细胞完全脱细胞，胶原纤维或弹性纤维完整保留，呈现典型的三维网络结构。兔角膜成纤维细胞可在材料上体外扩增。未观察到明显的排斥反应，材料逐渐被吸收。
胰蛋白酶 - 脱氧核糖核酸酶 - 核糖核酸酶法制备的脱细胞猪角膜基质材料适用于组织工程角膜的重建。
