Yi Xionghai, Lu Yitong
Department of Resource and Environmental Science, Shanghai Jiao Tong University, 2678 Qi Xin Road, Shanghai 201101, People's Republic of China.
Chemosphere. 2006 Oct;65(4):639-43. doi: 10.1016/j.chemosphere.2006.01.066. Epub 2006 Mar 10.
The simple and efficient method for determination of probenazole in soil, rice plant, and paddy water was developed, and the fate of probenazole in rice field ecosystem was also studied. Probenazole residues were extracted from sample, cleaned up by liquid/liquid partition and chromatographic column and then determined by gas chromatography with flame photometric detection. As far as the accuracy and precision was concerned, the method met certain standard. The LODs of probenazole calculated as a sample concentration (S/N ratio of 3) was 0.02 mg kg-1. The minimum detectable limit was 5x10(-10) g. The degradation of probenazole in soil, rice straw, and water was determined. The results showed that probenazole degradation in soil and rice straw coincided with C=0.576e-0.147t, C=17.858e-0.414t, respectively; the half-lives were about 4.7 and 1.7 d, respectively. The degradation rate of probenazole in rice straw was faster than that of in soil. Probenazole residue at 0.02 mg kg-1 could only be detected in paddy water within the first day after application. The final probenazole residues in soil, brown rice, and water were undetectable at levels of recommended and doubled dosage with an interval of 63 d. Therefore, a dosage of 1800-3600 g a.i. hm-2 was recommended, which could be considered as safe to human beings and animals. These would contribute to provide the scientific basis of using this fungicide.
建立了一种简单高效的测定土壤、水稻植株和稻田水中烯丙苯噻唑的方法，并研究了烯丙苯噻唑在稻田生态系统中的归宿。从样品中提取烯丙苯噻唑残留，通过液液分配和色谱柱净化，然后用火焰光度检测气相色谱法测定。就准确度和精密度而言，该方法符合一定标准。以样品浓度计算（信噪比为3），烯丙苯噻唑的检出限为0.02 mg kg-1。最低检测限为5×10(-10) g。测定了烯丙苯噻唑在土壤、稻草和水中的降解情况。结果表明，烯丙苯噻唑在土壤和稻草中的降解分别符合C = 0.576e-0.147t、C = 17.858e-0.414t；半衰期分别约为4.7 d和1.7 d。烯丙苯噻唑在稻草中的降解速率比在土壤中的快。施药后第1天内，仅在稻田水中检测到0.02 mg kg-1的烯丙苯噻唑残留。在推荐剂量和加倍剂量下，间隔63 d后，土壤、糙米和水中的烯丙苯噻唑最终残留均未检出。因此，推荐使用1800 - 3600 g a.i. hm-2的剂量，该剂量对人和动物可视为安全。这些将有助于为使用这种杀菌剂提供科学依据。
