脂质体在声孔效应中的应用。
Application of liposomes to sonoporation.
作者信息
Wu Junru, Chen Di, Pepe Jason, Himberg Benjamin E, Ricń Mercedes
机构信息
Department of Physics, University of Vermont, Burlington, VT 05405-0125, USA.
出版信息
Ultrasound Med Biol. 2006 Mar;32(3):429-37. doi: 10.1016/j.ultrasmedbio.2005.11.019.
A method to prepare liposomes is presented. Liposomes made in our laboratory were characterized acoustically and optically. The phase velocity and attenuation of liposomes in suspension (concentration = 10(9)/mL) were measured, ranging from 2 to 14 MHz, using ultrasound spectroscopy. Anti-rabbit IgG conjugated with Alexafluor 647 was delivered into Jurkat cells in suspension, using the liposomes, by 10 % duty cycle ultrasound tonebursts of 2.2 MHz (the in situ spatial peak-pressure amplitude = 80 W/cm2) with an efficiency of 13 %. It has been experimentally shown that liposomes may be an alternative stable agent to Optison for delivering macromolecules into cells.
本文介绍了一种制备脂质体的方法。对我们实验室制备的脂质体进行了声学和光学表征。使用超声光谱法测量了悬浮液中脂质体（浓度 = 10⁹/mL）在2至14 MHz范围内的相速度和衰减。通过脂质体，利用2.2 MHz的10%占空比超声脉冲串（原位空间峰值压力幅度 = 80 W/cm²），以13%的效率将与Alexafluor 647偶联的抗兔IgG递送至悬浮的Jurkat细胞中。实验表明，脂质体可能是一种替代Optison的稳定试剂，用于将大分子递送至细胞中。
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