Song Kyoung Seob, Peterson Yuri K, Freidman Andrew, Blumer Joe B, Sato Motohiko, Lanier S M
Department of Pharmacology and Experimental Therapeutics, Louisiana State University Health Sciences Center, 1901 Perdido Street, New Orleans, LA 70112, USA.
FEBS Lett. 2006 Apr 3;580(8):1993-8. doi: 10.1016/j.febslet.2006.02.071. Epub 2006 Mar 6.
The G-protein regulatory (GPR) motif is a approximately 25 amino acid sequence that stabilizes the GDP-bound conformation of Gialpha. To identify additional GPR motifs, we expanded a motif-based search strategy and identified an additional 4 mammalian proteins (WAVE1-3, rat GHRH) and 10 plant proteins with candidate GPR motifs. The WAVE1 GPR peptide inhibited GTPgammaS binding to purified G-protein. Endogenous Gialpha and WAVE1 coimmunoprecipitated from brain lysates. A WAVE1-G-protein complex was also observed following transfection of COS7 cells with Gialpha3 and WAVE1. The docking of Gialpha within a WAVE1 scaffolding complex may facilitate dynamic cycling and/or targeting for efficient and localized control of actin polymerization.
G蛋白调节（GPR）基序是一段约25个氨基酸的序列，可稳定Gialpha与GDP结合的构象。为了鉴定其他GPR基序，我们扩展了基于基序的搜索策略，并鉴定出另外4种哺乳动物蛋白（WAVE1 - 3、大鼠生长激素释放激素）和10种具有候选GPR基序的植物蛋白。WAVE1 GPR肽抑制GTPγS与纯化的G蛋白结合。内源性Gialpha和WAVE1从脑裂解物中共免疫沉淀。在用Gialpha3和WAVE1转染COS7细胞后，也观察到了WAVE1 - G蛋白复合物。Gialpha在WAVE1支架复合物中的对接可能有助于动态循环和/或靶向，以实现对肌动蛋白聚合的高效和局部控制。
