Kumar Sandeep, Allen George C, Thompson William F
Department of Botany, North Carolina State University, Raleigh, NC 27695-7612, USA.
Trends Plant Sci. 2006 Apr;11(4):159-61. doi: 10.1016/j.tplants.2006.02.002. Epub 2006 Mar 10.
Zinc-finger endonucleases (ZFNs) make targeted double-stranded breaks in genomic DNA and, thus, stimulate recombination and repair processes at specific sites. ZFNs can now be harnessed to stimulate homologous recombination and gene targeting in plants, which represents a major step towards modifying the plant genome more precisely. ZFN-mediated gene targeting is likely to become a powerful tool for genome research and genetic engineering.
锌指核酸酶（ZFNs）可在基因组DNA中产生靶向双链断裂，从而刺激特定位点的重组和修复过程。现在可以利用锌指核酸酶来刺激植物中的同源重组和基因靶向，这是朝着更精确地修饰植物基因组迈出的重要一步。锌指核酸酶介导的基因靶向可能会成为基因组研究和基因工程的强大工具。
