Hunter R L, Strickland F
J Natl Cancer Inst. 1975 May;54(5):1157-63. doi: 10.1093/jnci/54.5.1157.
The covalent conjugation of fatty acid to a tumor cell membrane preparation transformed it from an antigen that enhanced tumor growth to one that suppressed it. A crude cell membrane preparation was made by sequential hypertonic and hypotonic salt extraction of tumor cells from a fibrosarcoma induced in hamsters by simian virus 40. The membranes were chemically conjugated with dodecanoic anhydride in 0.5 M carbonate buffer (pH 9.0). Injection of unmodified membranes 10 days before transplantation of live tumor cells produced clear-cut enhancement of the tumor growth rate. In contrast, injection of lipid-conjugated membranes in a similar dose and protocol suppressed tumor growth. The lymphoid proliferative reactions to the tumor cells as demonstrated by the histology of both the tumor and regional lymph nodes were consistent with the hypothesis that unmodified membranes stimulated the production of antibody which participated in the enhancement of tumor growth, and that lipid-conjugated membranes stimulated the production of cell-mediated immunity which suppressed this growth.
脂肪酸与肿瘤细胞膜制剂的共价结合将其从一种促进肿瘤生长的抗原转变为一种抑制肿瘤生长的抗原。通过对猿猴病毒40诱导的仓鼠纤维肉瘤中的肿瘤细胞依次进行高渗和低渗盐提取，制备了粗制细胞膜制剂。这些膜在0.5 M碳酸盐缓冲液（pH 9.0）中与十二酸酐进行化学结合。在移植活肿瘤细胞前10天注射未修饰的膜，可明显提高肿瘤生长速率。相比之下，以类似剂量和方案注射脂质结合的膜则抑制肿瘤生长。肿瘤和局部淋巴结的组织学结果所显示的对肿瘤细胞的淋巴增殖反应与以下假设一致：未修饰的膜刺激了参与促进肿瘤生长的抗体的产生，而脂质结合的膜刺激了抑制这种生长的细胞介导免疫的产生。
