Haen E, Liebl B, Lederer T, Pliska V
Walther-Straub-Institute of Pharmacology and Toxicology, München, FRG.
J Recept Res. 1991;11(1-4):129-40. doi: 10.3109/10799899109066394.
Peripheral mononuclear leukocytes (pMNL) bear a population of beta 2-adrenoceptors. Radioreceptor assays with (-)-125Iodocyanopindolol (125I-CYP) are often used to determine the expression of these hormone receptors under physiological and pathological conditions. Doubts on the occurrence of just one class of binding sites as well as the availability of new laboratory equipment prompted us to revise the procedure employed for investigation of these receptors. pMNL were harvested from venous human blood by density centrifugation with LymphopaqueR, LymphoprepR, or FicollR yielding immunologically distinct pMNL fractions. Receptor binding assays were performed semi-automatically with 125I-CYP in the range from 0.6-600 pmol/l. Analysis of the data (modified affinity spectra, Scatchard plot) revealed two classes of binding sites (high- and low affinity binding). The binding isotherms were sigmoidal in the concentration range from 0.6-3.0 pmol/l. Parameters estimated for the high affinity binding site may vary by a factor of 10, depending on the mathematical model employed.
外周血单个核白细胞（pMNL）存在一群β2-肾上腺素能受体。常用（-）-125I-碘氰吲哚洛尔（125I-CYP）进行放射受体分析，以确定在生理和病理条件下这些激素受体的表达情况。由于对是否仅存在一类结合位点存在疑问以及新实验室设备的可用性，促使我们修订了用于研究这些受体的程序。通过使用LymphopaqueR、LymphoprepR或FicollR进行密度离心从人静脉血中采集pMNL，得到免疫特性不同的pMNL组分。用125I-CYP在0.6 - 600 pmol/l范围内半自动进行受体结合分析。对数据的分析（修正亲和光谱、Scatchard图）显示存在两类结合位点（高亲和力结合和低亲和力结合）。在0.6 - 3.0 pmol/l的浓度范围内，结合等温线呈S形。根据所采用的数学模型，为高亲和力结合位点估计的参数可能相差10倍。
