Behl R, Sheoran N, Behl J, Vijh R K
Animal Genetics Division, National Bureau of Animal Genetic Resources, Karnal, Haryana, India.
J Anim Breed Genet. 2006 Apr;123(2):131-5. doi: 10.1111/j.1439-0388.2006.00568.x.
Ankamali pigs, the domesticated native pigs of Kerala province of India were genetically characterized using 23 FAO recommended microsatellite markers and were compared with other native Indian pig types and Large White pigs. Twenty-six blood samples were collected from genetically unrelated animals from their breeding tract and DNA was isolated by standard procedure of phenol/chloroform. The genomic DNA was amplified by polymerase chain reaction (PCR) at these 23 microsatellite loci, which were also used earlier to characterize Desi (North Indian) and Gahuri (North-East Indian) pigs, the other two native domesticated pig types of India. The PCR products were resolved by denaturing urea-polyacrylamide gel electrophoresis and alleles were visualized after silver nitrate staining. The data were analysed for allele size range, number of alleles, allelic frequencies, heterozygosity and polymorphism information content for each locus. The allele size range varied between 92-108 bp at locus S0026 and 280-296 bp at locus IGF-1. The total number of alleles varied between five (S0178 and S0386) and 11 (S0355). The mean observed and expected heterozygosities were found to be 0.74 +/- 0.09 and 0.83 +/- 0.03 respectively. In the neighbour-joining dendrogram based on D(A) genetic distances developed after 1000 bootstraps, the Ankamali pigs did not show genetic closeness either with other native Indian pig types or exotic Large White pigs with high bootstrap values indicating genetic distinctness of Ankamali pigs.
安卡马利猪是印度喀拉拉邦的本土驯化猪种，利用23个联合国粮农组织推荐的微卫星标记对其进行了基因特征分析，并与其他印度本土猪种和大白猪进行了比较。从其繁殖地的非亲缘动物中采集了26份血样，采用苯酚/氯仿标准程序分离DNA。在这23个微卫星位点上通过聚合酶链反应（PCR）扩增基因组DNA，这些位点此前也曾用于鉴定印度另外两种本土驯化猪种德西猪（印度北部）和加胡里猪（印度东北部）。PCR产物通过变性尿素-聚丙烯酰胺凝胶电泳进行分离，硝酸银染色后观察等位基因。分析了每个位点的等位基因大小范围、等位基因数量、等位基因频率、杂合度和多态性信息含量。在S0026位点，等位基因大小范围在92 - 108 bp之间，在IGF - 1位点为280 - 296 bp。等位基因总数在5个（S0178和S0386）到11个（S0355）之间。观察到的平均杂合度和预期杂合度分别为0.74±0.09和0.83±0.03。在基于1000次自展后得出的D(A)遗传距离构建的邻接树状图中，安卡马利猪与其他印度本土猪种或外来的大白猪均未表现出遗传亲近性，较高的自展值表明安卡马利猪具有遗传独特性。
